@article{open2002,
          volume = {68},
          number = {3},
           month = {January},
          author = {Harjodh Singh and Manpreet Kaur and Lakhwinder Kaur and Shivani Sharma and Sunita Mishra and Naga Radha Srinivas Tanuku and Anil Kumar Pinnaka},
            note = {Copyright of this article belongs to Society for General Microbiology.},
           title = {Bacillus lacus sp. nov., isolated from a water sample of a salt lake in India},
       publisher = {Society for General Microbiology},
            year = {2018},
         journal = { International Journal of Systematic and Evolutionary Microbiology},
           pages = {801--809},
             url = {http://crdd.osdd.net/open/2002/},
        abstract = {A strictly aerobic, alkaliphilic, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK74T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India. Colonies were circular, 1.2 mm in diameter, shiny, smooth, whitish and convex with an entire margin after 48 h growth at 37 ?C with pH 9.0. Growth occurred at 25-42 ?C, 0-4 \% (w/v) NaCl and at a pH of 7-12. Strain AK74T was positive for aesculinase, caseinase, lipase activities and negative for oxidase, catalase, amylase, cellulase, DNase, gelatinase and urease activities. The fatty acids were dominated by branched iso-, anteiso- and saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The DNA G+C content of strain AK74T was 51.6 mol\%. A blast sequence similarity search based on 16S rRNA gene sequences indicated that Bacillus niabensis, Bacillus idriensisand Bacillus halosaccharovorans were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 96.6, 96.6 and 96.5\%, respectively. Phylogenetic analysis showed that strain AK74T clustered with Bacillus mangrove and together clustered with Bacillus idriensisand Bacillus indicus. Based on its phenotypic characteristics and on phylogenetic inference, strain AK74T represents a novel species of the genus Bacillus, for which the name Bacilluslacus sp. nov. is proposed. The type strain is AK74T (=MTCC 12638T=KCTC 33946T=JCM 32185T).}
}