%A Harjodh Singh
%A Manpreet Kaur
%A Lakhwinder Kaur
%A Shivani Sharma
%A Sunita Mishra
%A Naga Radha Srinivas Tanuku
%A Anil Kumar Pinnaka
%O Copyright of this article belongs to Society for General Microbiology.
%J  International Journal of Systematic and Evolutionary Microbiology
%T Bacillus lacus sp. nov., isolated from a water sample of a salt lake in India
%X A strictly aerobic, alkaliphilic, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK74T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India. Colonies were circular, 1.2?mm in diameter, shiny, smooth, whitish and convex with an entire margin after 48?h growth at 37??C with pH 9.0. Growth occurred at 25-42??C, 0-4?% (w/v) NaCl and at a pH of 7-12. Strain AK74T was positive for aesculinase, caseinase, lipase activities and negative for oxidase, catalase, amylase, cellulase, DNase, gelatinase and urease activities. The fatty acids were dominated by branched iso-, anteiso- and saturated fatty acids with a high abundance of iso-C15?:?0, anteiso-C15?:?0, C16?:?0 and C16?:?1 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The DNA G+C?content of strain AK74T was 51.6?mol%. A blast sequence similarity search based on 16S rRNA gene sequences indicated that Bacillus niabensis, Bacillus idriensisand Bacillus halosaccharovorans were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 96.6, 96.6 and 96.5%, respectively. Phylogenetic analysis showed that strain AK74T clustered with Bacillus mangrove and together clustered with Bacillus idriensisand Bacillus indicus. Based on its phenotypic characteristics and on phylogenetic inference, strain AK74T represents a novel species of the genus Bacillus, for which the name Bacilluslacus sp. nov. is proposed. The type strain is AK74T (=MTCC 12638T=KCTC 33946T=JCM 32185T).
%N 3
%P 801-809
%V 68
%D 2018
%I Society for General Microbiology
%R doi:10.1099/ijsem.0.002588
%L open2002