@article{open2015, volume = {68}, number = {2}, month = {January}, author = {Manpreet Kaur and Harjodh Singh and Shivani Sharma and Sunita Mishra and Naga Radha Srinivas Tanuku and Anil Kumar Pinnaka}, note = {Copyright of this article belongs to Society for General Microbiology.}, title = {Sphingobacterium bovisgrunnientis sp. nov., isolated from yak milk}, publisher = {Society for General Microbiology}, year = {2018}, journal = { International Journal of Systematic and Evolutionary Microbiology}, pages = {636--642}, keywords = {Gram-negative; Sphingobacterium; novel species; yak milk}, url = {http://crdd.osdd.net/open/2015/}, abstract = {A novel Gram-negative, rod shaped, non-motile bacterium, designated strain YK2T, was isolated from yak milk from Leh, India. The strain was positive for oxidase- and catalase-activities and negative for starch hydrolysis, nitrate reduction, citrate utilization, urease, lysine decarboxylase and ornithine decarboxylase activities. The predominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C17 : 1{\ensuremath{\omega}}9c and C16 : 1{\ensuremath{\omega}}7c and/or C16 : 1{\ensuremath{\omega}}6c and/or iso-C15 : 0 2-OH (summed feature 3). The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid and six unidentified lipids. The DNA G+C content of the strain was 38.9 mol\%. The 16S rRNA gene sequence analysis indicated that strain YK2T was a member of the genus Sphingobacterium and closely related to Sphingobacterium alimentarium and Sphingobacterium composti with pair-wise sequence similarity of 98.3 and 97.9 \%, respectively. The sequence similarity to other members of the genus Sphingobacterium was between 92.6 to 96.3 \%. Phylogenetic analysis showed that strain YK2T clustered with Sphingobacterium alimentarium and together clustered with Sphingobacterium composti. DNA-DNA hybridization of strain YK2T with Sphingobacterium alimentarium WCC 4521T and Sphingobacterium composti T5-12T showed a relatedness of only 38 and 54 \%, respectively. Based on the phenotypic characteristics and on phylogenetic inference, it appears that strain YK2T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium bovisgrunnientis sp. nov. is proposed. The type strain of Sphingobacterium bovisgrunnientis sp. nov. is YK2T (=MTCC 12631T=KCTC 52685T=JCM 31951T).} }