%A Manpreet Kaur
%A Harjodh Singh
%A Shivani Sharma
%A Sunita Mishra
%A Naga Radha Srinivas Tanuku
%A Anil Kumar Pinnaka
%O Copyright of this article belongs to Society for General Microbiology.
%J  International Journal of Systematic and Evolutionary Microbiology
%T Sphingobacterium bovisgrunnientis sp. nov., isolated from yak milk
%X A novel Gram-negative, rod shaped, non-motile bacterium, designated strain YK2T, was isolated from yak milk from Leh, India. The strain was positive for oxidase- and catalase-activities and negative for starch hydrolysis, nitrate reduction, citrate utilization, urease, lysine decarboxylase and ornithine decarboxylase activities. The predominant fatty acids were iso-C15?:?0, iso-C17?:?0 3-OH, iso-C17?:?1?9c and C16?:?1?7c and/or C16?:?1?6c and/or iso-C15?:?0 2-OH (summed feature 3). The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid and six unidentified lipids. The DNA G+C?content of the strain was 38.9?mol%. The 16S rRNA gene sequence analysis indicated that strain YK2T was a member of the genus Sphingobacterium and closely related to Sphingobacterium alimentarium and Sphingobacterium composti with pair-wise sequence similarity of 98.3 and 97.9?%, respectively. The sequence similarity to other members of the genus Sphingobacterium was between 92.6 to 96.3?%. Phylogenetic analysis showed that strain YK2T clustered with Sphingobacterium alimentarium and together clustered with Sphingobacterium composti. DNA-DNA hybridization of strain YK2T with Sphingobacterium alimentarium WCC 4521T and Sphingobacterium composti T5-12T showed a relatedness of only 38 and 54?%, respectively. Based on the phenotypic characteristics and on phylogenetic inference, it appears that strain YK2T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium bovisgrunnientis sp. nov. is proposed. The type strain of Sphingobacterium bovisgrunnientis sp. nov. is YK2T (=MTCC 12631T=KCTC 52685T=JCM 31951T).
%N 2
%K Gram-negative; Sphingobacterium; novel species; yak milk
%P 636-642
%V 68
%D 2018
%I Society for General Microbiology
%R doi:10.1099/ijsem.0.002562
%L open2015