creators_name: Kaur, Manpreet
creators_name: Singh, Harjodh
creators_name: Sharma, Shivani
creators_name: Mishra, Sunita
creators_name: Tanuku, Naga Radha Srinivas
creators_name: Pinnaka, Anil Kumar
type: article
datestamp: 2018-03-27 05:01:59
lastmod: 2019-03-20 12:22:46
metadata_visibility: show
title: Sphingobacterium bovisgrunnientis sp. nov., isolated from yak milk
ispublished: pub
subjects: QR
keywords: Gram-negative; Sphingobacterium; novel species; yak milk
note: Copyright of this article belongs to Society for General Microbiology.
abstract: A novel Gram-negative, rod shaped, non-motile bacterium, designated strain YK2T, was isolated from yak milk from Leh, India. The strain was positive for oxidase- and catalase-activities and negative for starch hydrolysis, nitrate reduction, citrate utilization, urease, lysine decarboxylase and ornithine decarboxylase activities. The predominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C17 : 1ω9c and C16 : 1ω7c and/or C16 : 1ω6c and/or iso-C15 : 0 2-OH (summed feature 3). The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid and six unidentified lipids. The DNA G+C content of the strain was 38.9 mol%. The 16S rRNA gene sequence analysis indicated that strain YK2T was a member of the genus Sphingobacterium and closely related to Sphingobacterium alimentarium and Sphingobacterium composti with pair-wise sequence similarity of 98.3 and 97.9 %, respectively. The sequence similarity to other members of the genus Sphingobacterium was between 92.6 to 96.3 %. Phylogenetic analysis showed that strain YK2T clustered with Sphingobacterium alimentarium and together clustered with Sphingobacterium composti. DNA-DNA hybridization of strain YK2T with Sphingobacterium alimentarium WCC 4521T and Sphingobacterium composti T5-12T showed a relatedness of only 38 and 54 %, respectively. Based on the phenotypic characteristics and on phylogenetic inference, it appears that strain YK2T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium bovisgrunnientis sp. nov. is proposed. The type strain of Sphingobacterium bovisgrunnientis sp. nov. is YK2T (=MTCC 12631T=KCTC 52685T=JCM 31951T).
date: 2018-01-08
date_type: published
publication:  International Journal of Systematic and Evolutionary Microbiology
volume: 68
number: 2
publisher: Society for General Microbiology
pagerange: 636-642
id_number: doi:10.1099/ijsem.0.002562
refereed: TRUE
issn: 1466-5026
official_url: http://dx.doi.org/10.1099/ijsem.0.002562
citation:   Kaur, Manpreet and Singh, Harjodh and Sharma, Shivani and Mishra, Sunita and Tanuku, Naga Radha Srinivas and Pinnaka, Anil Kumar  (2018) Sphingobacterium bovisgrunnientis sp. nov., isolated from yak milk.   International Journal of Systematic and Evolutionary Microbiology, 68 (2).  pp. 636-642.  ISSN 1466-5026