%0 Journal Article
%@ 2053-230X
%A Kumar, A
%A Karthikeyan, Subramanian
%D 2018
%F open:2016
%I John Wiley & Sons Inc
%J Acta Crystallographica Section F Structural Biology Communications
%K MSMEG_4306; Mycobacterium smegmatis; Rv2229c; X-ray crystallography; coiled-coil helix; zinc SAD; zinc ribbon
%N 3
%P 166-173
%T Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis 
%U http://crdd.osdd.net/open/2016/
%V 74
%X The MSMEG_4306 gene from Mycobacterium smegmatis encodes a protein of unknown function with 242 amino-acid residues that contains a conserved zinc-ribbon domain at its C-terminus. Here, the crystal structure of MSMEG_4306 determined by the single-wavelength anomalous dispersion method using just one zinc ion co-purified with the protein is reported. The crystal structure of MSMEG_4306 shows a coiled-coil helix domain in the N-terminal region and a zinc-ribbon domain in the C-terminal region. A structural similarity search against the Protein Data Bank using MSMEG_4306 as a query revealed two similar structures, namely CT398 from Chlamydia trachomatis and HP0958 from Helicobacter pylori, although they share only ∼15% sequence identity with MSMEG_4306. Based on comparative analysis, it is predicted that MSMEG_4306 may be involved in secretion systems, possibly by interacting with multiple proteins or nucleic acids.
%Z Copyright of this article belongs to John Wiley & Sons Inc.