TY  - JOUR
N1  - Copyright of this article belongs to John Wiley & Sons Inc.
ID  - open2016
UR  - http://dx.doi.org/10.1107/S2053230X18002236
IS  - 3
A1  - Kumar, A
A1  - Karthikeyan, Subramanian
Y1  - 2018/03/01/
N2  - The MSMEG_4306 gene from Mycobacterium smegmatis encodes a protein of unknown function with 242 amino-acid residues that contains a conserved zinc-ribbon domain at its C-terminus. Here, the crystal structure of MSMEG_4306 determined by the single-wavelength anomalous dispersion method using just one zinc ion co-purified with the protein is reported. The crystal structure of MSMEG_4306 shows a coiled-coil helix domain in the N-terminal region and a zinc-ribbon domain in the C-terminal region. A structural similarity search against the Protein Data Bank using MSMEG_4306 as a query revealed two similar structures, namely CT398 from Chlamydia trachomatis and HP0958 from Helicobacter pylori, although they share only ?15% sequence identity with MSMEG_4306. Based on comparative analysis, it is predicted that MSMEG_4306 may be involved in secretion systems, possibly by interacting with multiple proteins or nucleic acids.
PB  - John Wiley & Sons Inc
JF  - Acta Crystallographica Section F Structural Biology Communications
VL  - 74
KW  - MSMEG_4306; Mycobacterium smegmatis; Rv2229c; X-ray crystallography; coiled-coil helix; zinc SAD; zinc ribbon
SN  - 2053-230X
TI  - Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis 
SP  - 166
EP  - 173
ER  -