creators_name: Kumar, A
creators_name: Karthikeyan, Subramanian
type: article
datestamp: 2018-03-27 05:04:04
lastmod: 2019-03-20 12:33:36
metadata_visibility: show
title: Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis 
ispublished: pub
subjects: QR
keywords: MSMEG_4306; Mycobacterium smegmatis; Rv2229c; X-ray crystallography; coiled-coil helix; zinc SAD; zinc ribbon
note: Copyright of this article belongs to John Wiley & Sons Inc.
abstract: The MSMEG_4306 gene from Mycobacterium smegmatis encodes a protein of unknown function with 242 amino-acid residues that contains a conserved zinc-ribbon domain at its C-terminus. Here, the crystal structure of MSMEG_4306 determined by the single-wavelength anomalous dispersion method using just one zinc ion co-purified with the protein is reported. The crystal structure of MSMEG_4306 shows a coiled-coil helix domain in the N-terminal region and a zinc-ribbon domain in the C-terminal region. A structural similarity search against the Protein Data Bank using MSMEG_4306 as a query revealed two similar structures, namely CT398 from Chlamydia trachomatis and HP0958 from Helicobacter pylori, although they share only ∼15% sequence identity with MSMEG_4306. Based on comparative analysis, it is predicted that MSMEG_4306 may be involved in secretion systems, possibly by interacting with multiple proteins or nucleic acids.
date: 2018-03-01
date_type: published
publication: Acta Crystallographica Section F Structural Biology Communications
volume: 74
number: 3
publisher: John Wiley & Sons Inc
pagerange: 166-173
id_number: doi:10.1107/S2053230X18002236
refereed: TRUE
issn: 2053-230X
official_url: http://dx.doi.org/10.1107/S2053230X18002236
citation:   Kumar, A and Karthikeyan, Subramanian  (2018) Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis.  Acta Crystallographica Section F Structural Biology Communications, 74 (3).  pp. 166-173.  ISSN 2053-230X