%A Anup Kumar Ojha
%A Ashish Verma
%A Yash Pal
%A Deepak Bhatt
%A Shanmugam Mayilraj
%A Srinivasan Krishnamurthi
%O Copyright of this article belongs to Society for General Microbiology.
%J International Journal of Systematic and Evolutionary Microbiology
%T Marinomonas epiphytica sp. nov., isolated from a marine intertidal macroalga
%X A novel Gram-stain-negative, aerobic marine bacterial strain, SAB-3T, was isolated from brown macroalgae (Dictyota sp.) growing in the Arabian sea, Goa, India. The strain grew optimally at 30??C, with 2.0-4.0?% (w/v) NaCl and at pH 7.0 on marine agar medium. Strain SAB-3T was unable to hydrolyse aesculin and did not grow in the presence of rifamycin but showed resistance to antibiotics such as cefadroxil and co-trimoxazole. The major fatty acids were summed feature 8 (C18?:?1?7c/C18?:?1?6c), summed feature 3 (C16?:?1?7c/C16?:?1?6c) and C16?:?0, and Q-8 was the major ubiquinone. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 41.0?mol%. 16S rRNA gene sequencing and phylogenetic analysis indicated that the strain was a member of the genus Marinomonas with Marinomonas aquiplantarum IVIA-Po-159T (97.6?% similarity), Marinomonas posidonica IVIA-Po-181T (97.5?%) and Marinomonas dokdonensis DSM 17202T (97.4?%) as the closest relatives. Whole genome relatedness determined through DNA-DNA hybridization revealed values of 40-50?% (below the 70?% threshold recommended for species delineation) with the above three species, thus confirming it as representing a distinct and novel species of the genus Marinomonas for which the name Marinomonas epiphytica sp. nov. is proposed. The type strain is SAB-3T (=JCM 31365T=KCTC 52293T=MTCC 12569T).
%N 8
%K Bacterial Typing Techniques
Base Composition
DNA, Bacterial/genetics;Fatty Acids/chemistry;
%P 2746-2751
%V 67
%D 2017
%I Society for General Microbiology
%R doi:10.1099/ijsem.0.002014
%L open2049