TY  - JOUR
N1  - Copyright of this article belongs to Elsevier.
ID  - open2063
UR  - http://dx.doi.org/10.1016/j.bbrc.2017.07.125
IS  - 3
A1  - Mahajan, Sonal
A1  - Khairnar, Aasawari
A1  - Bishnoi, Ritika
A1  - Ramya, T.N.C.
Y1  - 2017///
N2  - F-type lectins are fucose binding lectins with characteristic fucose binding and calcium binding motifs. Although they occur with a selective distribution in viruses, prokaryotes and eukaryotes, most biochemical studies have focused on vertebrate F-type lectins. Recently, using sensitive bioinformatics search techniques on the non-redundant database, we had identified many microbial F-type lectin domains with diverse domain organizations. We report here the biochemical characterization of F-type lectin domains from Cyanobium sp. PCC 7001, Myxococcus hansupus and Leucothrix mucor. We demonstrate that while all these three microbial F-type lectin domains bind to the blood group H antigen epitope on fucosylated glycans, there are fine differences in their glycan binding specificity. Cyanobium sp. PCC 7001 F-type lectin domain binds exclusively to extended H type-2 motif, Myxococcus hansupus F-type lectin domain binds to B, H type-1 and Lewisb motifs, and Leucothrix mucor F-type lectin domain binds to a wide range of fucosylated glycans, including A, B, H and Lewis antigens. We believe that these microbial lectins will be useful additions to the glycobiologist's toolbox for labeling, isolating and visualizing glycans.
PB  - Elsevier
JF  - Biochemical and Biophysical Research Communications
VL  - 491
KW  - Blood group antigens; Cyanobium sp. FLD; F-type lectin domain; Fucose; Leucothrix mucor FLD; Myxococcus hansupus FLD
SN  - 0006291X
TI  - Microbial F-type lectin domains with affinity for blood group antigens
SP  - 708
EP  - 713
ER  -