creators_name: Mahajan, Sonal
creators_name: Khairnar, Aasawari
creators_name: Bishnoi, Ritika
creators_name: Ramya, T.N.C.
type: article
datestamp: 2018-03-28 05:55:03
lastmod: 2018-03-28 05:55:03
metadata_visibility: show
title: Microbial F-type lectin domains with affinity for blood group antigens
ispublished: pub
subjects: QR
keywords: Blood group antigens; Cyanobium sp. FLD; F-type lectin domain; Fucose; Leucothrix mucor FLD; Myxococcus hansupus FLD
note: Copyright of this article belongs to Elsevier.
abstract: F-type lectins are fucose binding lectins with characteristic fucose binding and calcium binding motifs. Although they occur with a selective distribution in viruses, prokaryotes and eukaryotes, most biochemical studies have focused on vertebrate F-type lectins. Recently, using sensitive bioinformatics search techniques on the non-redundant database, we had identified many microbial F-type lectin domains with diverse domain organizations. We report here the biochemical characterization of F-type lectin domains from Cyanobium sp. PCC 7001, Myxococcus hansupus and Leucothrix mucor. We demonstrate that while all these three microbial F-type lectin domains bind to the blood group H antigen epitope on fucosylated glycans, there are fine differences in their glycan binding specificity. Cyanobium sp. PCC 7001 F-type lectin domain binds exclusively to extended H type-2 motif, Myxococcus hansupus F-type lectin domain binds to B, H type-1 and Lewisb motifs, and Leucothrix mucor F-type lectin domain binds to a wide range of fucosylated glycans, including A, B, H and Lewis antigens. We believe that these microbial lectins will be useful additions to the glycobiologist's toolbox for labeling, isolating and visualizing glycans.
date: 2017
date_type: published
publication: Biochemical and Biophysical Research Communications
volume: 491
number: 3
publisher: Elsevier
pagerange: 708-713
id_number: doi:10.1016/j.bbrc.2017.07.125
refereed: TRUE
issn: 0006291X
official_url: http://dx.doi.org/10.1016/j.bbrc.2017.07.125
citation:   Mahajan, Sonal and Khairnar, Aasawari and Bishnoi, Ritika and Ramya, T.N.C.  (2017) Microbial F-type lectin domains with affinity for blood group antigens.  Biochemical and Biophysical Research Communications, 491 (3).  pp. 708-713.  ISSN 0006291X