@article{open2073, volume = {67}, number = {9}, author = {Chandni Sidhu and Suresh Thakur and Gunjan Sharma and Naga Radha Srinivas Tanuku and Anil Kumar Pinnaka}, note = {Copyright of this article belongs to SGM}, title = {Oceanospirillum sanctuarii sp. nov., isolated from a sediment sample}, publisher = {Society for General Microbiology}, year = {2017}, journal = {International Journal of Systematic and Evolutionary Microbiology}, pages = {3428--3434}, url = {http://crdd.osdd.net/open/2073/}, abstract = {A novel Gram-staining-negative, spiral-shaped, pale-yellow, non-sporulating, motile, aerobic bacterium, designated strain AK56T, was isolated from a sediment sample collected at the Coringa Wildlife Sanctuary, India. Colonies on marine agar were circular, pale yellow, shiny, translucent, 1-2 mm in diameter, convex and had an entire margin. The major fatty acids included C16 : 1, C16 : 1{\ensuremath{\omega}}7c/C16 : 1{\ensuremath{\omega}}6c and C18 : 1{\ensuremath{\omega}}7c. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, one unidentified phospholipid and five unidentified lipids. DNA-DNA hybridization between strain AK56T and Oceanospirillum linum LMG 5214T and 'Oceanospirillum nioense ' NIO-S6 showed relatedness values of 39.91 and 23.62 \%, respectively. The DNA G+C content of strain AK56T was found to be 50.3 mol\%. A sequence similarity search for the 16S rRNA gene sequence revealed that O. linum and O. nioense were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 98.9 and 98.2 \%, respectively. Phylogenetic analysis also showed the formation of a cluster including strain AK56T with close relative O. linum and O. nioense. Based on the observed phenotypic, chemotaxonomic characteristics and phylogenetic analysis, strain AK56T is described in this study as a novel species in the genus Oceanospirillum, for which the name Oceanospirillum sanctuarii sp. nov. is proposed. The type strain of Oceanospirillumsanctuarii is AK56T (=MTCC 12005T=JCM 19193T=KCTC 52973T).} }