creators_name: Sidhu, Chandni
creators_name: Thakur, Suresh
creators_name: Sharma, Gunjan
creators_name: Tanuku, Naga Radha Srinivas
creators_name: Pinnaka, Anil Kumar
type: article
datestamp: 2018-03-28 06:35:29
lastmod: 2018-03-28 06:35:29
metadata_visibility: show
title: Oceanospirillum sanctuarii sp. nov., isolated from a sediment sample
ispublished: pub
subjects: QR
note: Copyright of this article belongs to SGM
abstract: A novel Gram-staining-negative, spiral-shaped, pale-yellow, non-sporulating, motile, aerobic bacterium, designated strain AK56T, was isolated from a sediment sample collected at the Coringa Wildlife Sanctuary, India. Colonies on marine agar were circular, pale yellow, shiny, translucent, 1-2 mm in diameter, convex and had an entire margin. The major fatty acids included C16 : 1, C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, one unidentified phospholipid and five unidentified lipids. DNA-DNA hybridization between strain AK56T and Oceanospirillum linum LMG 5214T and 'Oceanospirillum nioense ' NIO-S6 showed relatedness values of 39.91 and 23.62 %, respectively. The DNA G+C content of strain AK56T was found to be 50.3 mol%. A sequence similarity search for the 16S rRNA gene sequence revealed that O. linum and O. nioense were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 98.9 and 98.2 %, respectively. Phylogenetic analysis also showed the formation of a cluster including strain AK56T with close relative O. linum and O. nioense. Based on the observed phenotypic, chemotaxonomic characteristics and phylogenetic analysis, strain AK56T is described in this study as a novel species in the genus Oceanospirillum, for which the name Oceanospirillum sanctuarii sp. nov. is proposed. The type strain of Oceanospirillumsanctuarii is AK56T (=MTCC 12005T=JCM 19193T=KCTC 52973T).
date: 2017
date_type: published
publication: International Journal of Systematic and Evolutionary Microbiology
volume: 67
number: 9
publisher: Society for General Microbiology
pagerange: 3428-3434
id_number: doi:10.1099/ijsem.0.002132
refereed: TRUE
issn: 1466-5026
official_url: http://dx.doi.org/10.1099/ijsem.0.002132
citation:   Sidhu, Chandni and Thakur, Suresh and Sharma, Gunjan and Tanuku, Naga Radha Srinivas and Pinnaka, Anil Kumar  (2017) Oceanospirillum sanctuarii sp. nov., isolated from a sediment sample.  International Journal of Systematic and Evolutionary Microbiology, 67 (9).  pp. 3428-3434.  ISSN 1466-5026