@article{open2075,
          volume = {67},
          number = {7},
          author = {Vasundhera Gupta and Pradip Kumar Singh and Suresh Korpole and Naga Radha Srinivas Tanuku and Anil Kumar Pinnaka},
            note = {Copyright of this article belongs to SGM.},
           title = {Bacillus mangrovi sp. nov., isolated from a sediment sample from a mangrove forest},
       publisher = {Society for General Microbiology},
            year = {2017},
         journal = {International Journal of Systematic and Evolutionary Microbiology},
           pages = {2219--2224},
             url = {http://crdd.osdd.net/open/2075/},
        abstract = {A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5 mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48 h growth at 30 ?C. Growth occurred at 15-42 ?C, with 0-3 \% (w/v) NaCl and at pH 6-9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities. The fatty acids were dominated by branched types with iso- and anteiso- saturated fatty acids with a high abundance of iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0; the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid; and MK-7 was the major menaquinone. DNA-DNA hybridization between AK61T and Bacillus indicus MTCC 4374T and between AK61T and Bacillus indicus KCTC 3880 showed relatedness of 37.99 and 33.32 \% respectively. The DNA G+C content of AK61T was 44 mol\%. The results of a blast sequence similarity search based on 16S rRNA gene sequences indicated that Bacillus cibi and Bacillus indicus were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 97.69 and 97.55 \% respectively. The results of phylogenetic analysis indicated that AK61T was clustered with Bacillus idriensis and Bacillus indicus. On the basis of its phenotypic characteristics and phylogenetic inference, AK61T represents a novel species of the genus Bacillus, for which the name Bacillus mangrovi sp. nov. is proposed. The type strain is AK61T (=JCM 31087T=MTCC 12015T=KCTC 33872T).}
}