title: System wide analysis unravels differential regulation and in vivo essentiality of VapBC TA systems from Mycobacterium tuberculosis
creator: Agarwal, Sakshi
creator: Tiwari, Prabhakar
creator: Deep, Amar
creator: Kidwai, Saqib
creator: Gupta, Shamba
creator: Thakur, Krishan Gopal
creator: Singh, Ramandeep
subject: QR Microbiology
description: oxin-antitoxin (TA) systems are bicistronic genetic modules that are ubiquitously present in bacterial genomes. Mycobacterium tuberculosis (Mtb) genome encodes for 90 putative TA systems and these are considered to be associated with maintenance of bacterial genomic stability or its survival under unfavorable environment. Majority of these in Mtb have been annotated as belonging to the Virulence associated protein B and C (VapBC) family. However, their precise role in bacterial physiology has not been elucidated. Here, we functionally characterized VapC toxins from Mtb and show that overexpression of some homologs inhibits growth of M. bovis BCG in a bacteriostatic manner. Expression profiling of mRNA revealed that these VapC toxins were differentially induced upon exposure of Mtb to stress conditions. We also unraveled that transcriptional cross-activation exists between TA systems in Mtb. This study provides the first evidence for the essentiality of VapBC3 and VapBC4 systems in Mtb virulence.
publisher: OUP
date: 2018-05-05
type: Article
type: PeerReviewed
relation: http://dx.doi.org/10.1093/infdis/jiy109
identifier:   Agarwal, Sakshi and Tiwari, Prabhakar and Deep, Amar and Kidwai, Saqib and Gupta, Shamba and Thakur, Krishan Gopal and Singh, Ramandeep  (2018) System wide analysis unravels differential regulation and in vivo essentiality of VapBC TA systems from Mycobacterium tuberculosis.  The Journal of Infectious Diseases.   ISSN 0022-1899     
relation: http://crdd.osdd.net/open/2081/