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primary topic: Production, Purification, and Characterization of Thermostable Alkaline Xylanase From NASTPD13.

Production, Purification, and Characterization of Thermostable Alkaline Xylanase From NASTPD13.
type: AcademicArticle
type: Article
abstract: NASTPD13 used herein as a source for thermostable alkaline xylanase were isolated from Paudwar Hot Springs, Nepal. NASTPD13 cultured at 60°C, pH 7 and in presence of inorganic (ammonium sulfate) or organic (yeast extract) nitrogen sources, produced maximum xylanase enzyme. Xylanase production in the cultures was monitored by following the ability of culture media to hydrolyze beech wood xylan producing xylooligosaccharide and xylose by thin layer chromatography (TLC). The extracellular xylanase was isolated from optimized NASTPD13 cultures by ammonium sulfate (80%) precipitation; the enriched xylanase preparation was dialyzed and purified using Sephadex G100 column chromatography. The purified xylanaseshowed 11-fold enrichment with a specific activity of 33 U/mg and molecular weight were37 kDa based on SDS-PAGE and PAGE-Zymography. The optimum pH and temperature of purified xylanase was 9.0 and 65°C respectively retainingmore than 50% of its maximal activity over a broad range of pH (6-9) and temperature (30-65°C). With beech wood xylan, the enzyme showed Km 0.7 mg/ml and Vmax 66.64 μM/min/mg The xylanase described herein is a secretory enzyme produced in large quantities by NASTPD13 and is a novel thermostable, alkaline xylanase with potential biotechnological applications.
date: 2018-05-15
volume: 6
is part of: Frontiers in bioengineering and biotechnology
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

Frontiers Media S.A.
type: Organization

Frontiers in bioengineering and biotechnology
type: Collection
issn: 22964185

Jyoti Maharjan
type: Person
given name: Jyoti
family name: Maharjan

Suresh Korpole
type: Person
given name: Suresh
family name: Korpole

Lakshmaiah Sreerama
type: Person
given name: Lakshmaiah
family name: Sreerama

Gandham S Prasad
type: Person
given name: Gandham S
family name: Prasad

Punam Yadav
type: Person
given name: Punam
family name: Yadav

Girish Sahni
type: Person
given name: Girish
family name: Sahni

Tribikram Bhattarai
type: Person
given name: Tribikram
family name: Bhattarai

HTML Summary of #2094 Production, Purification, and Characterization of Thermostable Alkaline Xylanase From NASTPD13.
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primary topic: Production, Purification, and Characterization of Thermostable Alkaline Xylanase From NASTPD13.

QR Microbiology
type: Concept