creators_name: Sudan, Sarabjeet Kour
creators_name: Kumar, Narender
creators_name: Kaur, Ishwinder
creators_name: Sahni, Girish
type: article
datestamp: 2018-08-09 06:28:55
lastmod: 2018-08-09 06:28:55
metadata_visibility: show
title: Production, purification and characterization of raw starch hydrolyzing thermostable acidic α-amylase from hot springs, India.
ispublished: pub
subjects: QR
keywords: Geobacillus; Raw starch; Thermostability; α-Amylase
note: Copyright of this article belongs to Elsevier.
abstract: Alpha-amylase is an important hydrolytic enzyme used for various industrial processes. In the present study, Geobacillus bacterium (K1C), producing a thermostable α-amylase was isolated from Manikaran hot springs, India. We have purified and characterized the biochemical properties of α-amylase. The optimum temperature and pH for α-amylase activity was 80 °C and pH 6.0 respectively. The far-UV CD spectra of the enzyme indicated the presence of random coil conformation and showed an intermediate phase during temperature-induced unfolding. In the presence of substrate, thermostability of the α-amylase was increased as 50% initial activity was retained at 70 °C for 6 h and at 80 °C for 2 h. Moreover, the enzyme also showed remarkable pH stability as 90% of the initial activity was retained even after 48 h of incubation at pH 5.0, 6.0 and 7.0. Interestingly, amylase activity of the purified enzyme was Caindependent, whereas the complete inhibition of activity was observed in the presence of Cu, Pb, and Hg. The purified α-amylase was stable in the presence of detergents, organic solvents and Proteinase K. Furthermore, it exhibited the ability to hydrolyze raw starches (e.g. rice, wheat, corn, potato) efficiently; thus this enzyme has the potential to be used for industrial applications.
date: 2018-06-01
date_type: published
publication: International journal of biological macromolecules
volume: 117
publisher: Elsevier Science Ltd
pagerange: 831-839
refereed: TRUE
issn: 1879-0003
official_url: https://www.sciencedirect.com/science/article/pii/S0141813018312042?via%3Dihub
citation:   Sudan, Sarabjeet Kour and Kumar, Narender and Kaur, Ishwinder and Sahni, Girish  (2018) Production, purification and characterization of raw starch hydrolyzing thermostable acidic α-amylase from hot springs, India.  International journal of biological macromolecules, 117.  pp. 831-839.  ISSN 1879-0003