TY  - JOUR
ID  - open2106
UR  - http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002726#tab2
IS  - 5
A1  - Singh, Harjodh
A1  - Kaur, Manpreet
A1  - Sharma, Shivani
A1  - Kaur, Lakhwinder
A1  - Mishra, Sunita
A1  - Tanuku, Naga Radha Srinivas
A1  - Pinnaka, Anil Kumar
Y1  - 2018/05/01/
N2  - An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India. Colonies were circular, 1-2?mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48?h growth on marine agar at pH 9 and 37?°C. Growth occurred at 15-42?°C, 0-10?% (w/v) NaCl and at a pH range of 7-12. Strain AK73 was positive for catalase and arginine dihydrolase 2 activities, hydrolysis of Tweens 20, 40 and 80, and negative for esculinase, caseinase, gelatinase, ?-galactosidase, lipase (Tween 60) and urease activities. The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15?:?0, anteiso-C15?:?0, C16?:?0 and anteiso-C17?:?0; MK-7 was the major menaquinone. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, four unidentified phospholipids and three unidentified lipids. The DNA G+C?content of strain AK73 was 54?mol%. Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0?%. Phylogenetic analysis showed that strain AK73 formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii, Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4?%, respectively. Based on its phenotypic characteristics and on phylogenetic inference, strain AK73 represents a novel species of the genus Bacillus, for which the name Bacillus alkalilacus sp. nov. is proposed. The type strain is AK73 (=JCM 32184=MTCC 12637=KCTC 33880).
PB  - Microbiology Society
JF  - International journal of systematic and evolutionary microbiology
VL  - 68
KW  - BLAST
KW  -  Basic Local Alignment Search Tool; FAME
KW  -  fatty acid methyl ester; GC
KW  -  gas chromatography; MA
KW  -  marine agar.
SN  - 1466-5034
TI  - Bacillus alkalilacus sp. nov., isolated from a sediment sample from a lake in India.
SP  - 1665
EP  - 1671
ER  -