TY  - JOUR
N1  - Copyright of this article belongs to Elsevier Science.
ID  - open2114
UR  - https://doi.org/10.1016/j.snb.2018.02.033
IS  - 1
A1  - Singh, Pargat
A1  - Gupta, Ritika
A1  - Choudhury, Megha
A1  - Pinnaka, Anil Kumar
A1  - Kumar, Rajesh
A1  - Bhalla, Vijayender
Y1  - 2018/06/01/
N2  - Inspired by the interaction of colistin with lipopolysaccharides (LPS) of the bacterial outer membrane, we describe a simple, cost effective and rapid assay for the detection of bacterial contamination in water samples. Colistin, a bactericidal drug, has been used by us in a receptor configuration for detection of pathogenic microorganisms without involving any tedious sample preparation step. The approach employs the cationic antibiotic drug for dual purpose, firstly, as a primary binder for pathogens and secondly, as an aggregator for negatively charged Gold nanoparticles (GNPs). The former consists of colistin binding to bacteria in water that renders GNPs free in solution thus depicting red color and the latter shows colistin driven aggregation of GNPs producing blue colored solution. The assay works in a two-step procedure that involves addition of colistin and GNPs to the water sample before results can be visualized based on color change. The assay is sensitive at a concentration up to 10 bacterial cellsˇmL?1 in a time frame of 5?min without requiring any expensive reagents and instruments.
PB  - Elsevier Science Ltd
JF  - Sensors and Actuators B: Chemical
VL  - 262
KW  - ColistinVisual detectionGold nanoparticlesPathogen detection
TI  - Drug and nanoparticle-mediated rapid naked eye water test for pathogens detection
SP  - 603
EP  - 610
ER  -