creators_name: Singh, Pargat
creators_name: Gupta, Ritika
creators_name: Choudhury, Megha
creators_name: Pinnaka, Anil Kumar
creators_name: Kumar, Rajesh
creators_name: Bhalla, Vijayender
type: article
datestamp: 2018-08-23 06:43:42
lastmod: 2019-03-20 14:15:06
metadata_visibility: show
title: Drug and nanoparticle-mediated rapid naked eye water test for pathogens detection
ispublished: pub
subjects: QR
keywords: ColistinVisual detectionGold nanoparticlesPathogen detection
note: Copyright of this article belongs to Elsevier Science.
abstract: Inspired by the interaction of colistin with lipopolysaccharides (LPS) of the bacterial outer membrane, we describe a simple, cost effective and rapid assay for the detection of bacterial contamination in water samples. Colistin, a bactericidal drug, has been used by us in a receptor configuration for detection of pathogenic microorganisms without involving any tedious sample preparation step. The approach employs the cationic antibiotic drug for dual purpose, firstly, as a primary binder for pathogens and secondly, as an aggregator for negatively charged Gold nanoparticles (GNPs). The former consists of colistin binding to bacteria in water that renders GNPs free in solution thus depicting red color and the latter shows colistin driven aggregation of GNPs producing blue colored solution. The assay works in a two-step procedure that involves addition of colistin and GNPs to the water sample before results can be visualized based on color change. The assay is sensitive at a concentration up to 10 bacterial cells·mL−1 in a time frame of 5 min without requiring any expensive reagents and instruments.
date: 2018-06-01
date_type: published
publication: Sensors and Actuators B: Chemical
volume: 262
number: 1
publisher: Elsevier Science Ltd
pagerange: 603-610
id_number: https://doi.org/10.1016/j.snb.2018.02.033
refereed: TRUE
official_url: https://doi.org/10.1016/j.snb.2018.02.033
citation:   Singh, Pargat and Gupta, Ritika and Choudhury, Megha and Pinnaka, Anil Kumar and Kumar, Rajesh and Bhalla, Vijayender  (2018) Drug and nanoparticle-mediated rapid naked eye water test for pathogens detection.  Sensors and Actuators B: Chemical, 262 (1).  pp. 603-610.