<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Alanine-scanning mutagenesis of WH2 domains of VopF reveals residues important for conferring lethality in a Saccharomyces cerevisiae model."^^ . "VopF, the type III effector molecule, has been implicated in the pathogenesis of non-O1, non-O139 strains of Vibrio cholerae. It is a protein of 530 amino acids, comprises of one formin homology 1-like (FH1-like) domain and three WASP homology 2 (WH2) domains. Previous works have demonstrated that WH2 domains are crucial for VopF function as a modulator of cellular actin homeostasis. Furthermore, domain deletion analysis also suggests that VopF variant constituted with only WH2 domain 3 is more efficient in restricting the growth of budding yeast than its congeners containing either only domain 1 or domain 2. Interestingly, a good degree of sequence diversity is present within each WH2 domain of VopF. In order to ascertain the importance of different amino acids in each WH2 domain, a systemic alanine scanning mutagenesis was employed. Using a yeast model system, the alanine derivatives of each amino acid of WH2 domain 1 and 3 of VopF were examined for growth restricting activity. Taken together, our mutagenesis results reveal the identification of critical residues of WH2 domain 1 and 3 of VopF."^^ . "2013-08-01" . "525" . "1" . . "Elsevier Science Ltd"^^ . . . "Gene"^^ . . . "18790038" . . . . . . . . . . . . . . . . "Saumya"^^ . "Raychaudhuri"^^ . "Saumya Raychaudhuri"^^ . . "Vikas"^^ . "Kaithwas"^^ . "Vikas Kaithwas"^^ . . "Ranjana"^^ . "Tripathi"^^ . "Ranjana Tripathi"^^ . . "Chetna"^^ . "Dureja"^^ . "Chetna Dureja"^^ . . . . . "HTML Summary of #2118 \n\nAlanine-scanning mutagenesis of WH2 domains of VopF reveals residues important for conferring lethality in a Saccharomyces cerevisiae model.\n\n" . "text/html" . . . "QR Microbiology"@en . .