creators_name: Nag, Dhrubajyoti
creators_name: Breen, Paul
creators_name: Raychaudhuri, Saumya
creators_name: Withey, Jeffrey H
type: article
datestamp: 2018-10-04 10:20:18
lastmod: 2018-10-04 10:20:18
metadata_visibility: show
title: Glucose metabolism by  inhibits  intestinal colonization of zebrafish.
ispublished: pub
subjects: QR180
note: Copyright of this article belongs to  American Society for Microbiology.
abstract: The  O1 serogroup is responsible for pandemic cholera and is divided into the classical and El Tor biotypes. Classical  produces acid when using glucose as a carbon source, whereas El Tor  produces the neutral product acetoin when using glucose as a carbon source. An earlier study demonstrated that  strains that metabolize glucose to acidic by-products drastically reduced the survival of  strains in vitro. In the present study, zebrafish were fed 1% glucose and inoculated with single  or  strains or co-infected with both  and  A significant decrease in classical biotype colonization was observed after glucose feeding due to acid production in the zebrafish intestine. El Tor colonization was unaffected by glucose alone. However, the El Tor strain exhibited significantly lower colonization of the zebrafish when either of the acid producing  was co-inoculated in the presence of glucose. An  sugar transport mutant had no effect on  colonization even in presence of glucose. Glucose and  produced a prophylactic effect on El Tor colonization in zebrafish when  was inoculated before  infection. Thus, the probiotic feeding of  inhibits  colonization in a natural host. This suggests that a similar inhibitory effect could be seen in cholera patients, especially if glucose-based oral rehydration solution (ORS) is administered in combination with probiotic  during cholera treatment.
date: 2018-09-24
date_type: published
publication: Infection and immunity
publisher: American Society for Microbiology
refereed: TRUE
issn: 1098-5522
official_url: https://iai.asm.org/content/early/2018/09/18/IAI.00486-18.long
citation:   Nag, Dhrubajyoti and Breen, Paul and Raychaudhuri, Saumya and Withey, Jeffrey H  (2018) Glucose metabolism by inhibits intestinal colonization of zebrafish.  Infection and immunity.   ISSN 1098-5522