%A Preeti Pathaniya
%A Anurima Sharma
%A Binod Kumar
%A Praveen Rishi
%A C Raman Suri
%O Copyright of this article belongs to Springer.
%J Mcrichimica Acta
%T Selective identification of specific aptamers for the detection of non-typhoidal salmonellosis in an apta-impedimetric sensing format
%X The authors report on an aptamer-based method for the detection of S. Typhimurium. The aptamers were identified by using a modified cell-based SELEX method (cell-SELEX) by adopting an alternating negative-positive selection strategy. Each cross-reactive population was used separately, with a focus on generating strain-specific aptamers in order to enhance selectivity. The aptamers were characterized and chemically modified with thiol groups at the 5? end which enables covalent binding to gold nanoparticles on a screen printed carbon electrode (GNP-SPCE). The aptamer-modified GNP-SPCE was subsequently applied to impedimetric sensing of S. Typhimurium in water and food samples. The response is linear in the 10 to 105 CFU?mL?1 concentration range, and the limit of detection is ~10 CFU?mL?1. The assay can distinguish S. Typhimurium from S. Typhi and has been validated in water and spiked egg samples.
%N 5
%K S. Typhimurium; Modified cell-SELEX; Gold nanoparticles; Impedimetry Aptasensor; Microbial pathogen 
%P 1499-1508
%V 184
%D 2017
%I Springer
%L open2126