@article{open2129,
          volume = {8},
          number = {24},
           month = {December},
          author = {Gaurav Kumar and Subhash B Arya and Amit Tuli},
           title = {Method for Studying the Effect of Gene Silencing on Bacterial Infection-induced ERK1/2 Signaling in Bone-marrow Derived Macrophages.},
       publisher = {Bio-protocol },
         journal = {Bio-protocol},
            year = {2018},
        keywords = {Bone-marrow derived macrophages; ERK signaling; Infection; Salmonella; Western blotting},
             url = {http://crdd.osdd.net/open/2129/},
        abstract = {Macrophages are highly phagocytic cells that utilize various pathogen recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs). These PAMPs can be present within the microbe, such as bacterial CpG DNA, and are recognized by Toll-like receptor 9 (TLR9), a PRR present on the endosomal membrane of macrophages. PAMPs can also be present on the surface of microbes, such as Lipopolysaccharide (LPS), which decorates the outer membrane of gram-negative bacteria like  and . LPS is recognized by TLR4 present on the plasma membrane of macrophages, and LPS-TLR4 association leads to activation of signaling cascades including MAPK phosphorylation, which in turn promotes macrophage activation and microbial killing. This protocol describes the method for studying the role of a gene of interest in Extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) signaling, induced by bacterial infection in primary bone-marrow derived macrophages (BMDMs).}
}