creators_name: Kumar, Gaurav
creators_name: Arya, Subhash B
creators_name: Tuli, Amit
type: article
datestamp: 2019-03-19 15:24:53
lastmod: 2019-03-19 15:24:53
metadata_visibility: show
title: Method for Studying the Effect of Gene Silencing on Bacterial Infection-induced ERK1/2 Signaling in Bone-marrow Derived Macrophages.
ispublished: pub
subjects: QR
keywords: Bone-marrow derived macrophages; ERK signaling; Infection; Salmonella; Western blotting
abstract: Macrophages are highly phagocytic cells that utilize various pathogen recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs). These PAMPs can be present within the microbe, such as bacterial CpG DNA, and are recognized by Toll-like receptor 9 (TLR9), a PRR present on the endosomal membrane of macrophages. PAMPs can also be present on the surface of microbes, such as Lipopolysaccharide (LPS), which decorates the outer membrane of gram-negative bacteria like  and . LPS is recognized by TLR4 present on the plasma membrane of macrophages, and LPS-TLR4 association leads to activation of signaling cascades including MAPK phosphorylation, which in turn promotes macrophage activation and microbial killing. This protocol describes the method for studying the role of a gene of interest in Extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) signaling, induced by bacterial infection in primary bone-marrow derived macrophages (BMDMs).
date: 2018-12-20
date_type: published
publication: Bio-protocol
volume: 8
number: 24
publisher: Bio-protocol 
refereed: TRUE
issn: 2331-8325
citation:   Kumar, Gaurav and Arya, Subhash B and Tuli, Amit  (2018) Method for Studying the Effect of Gene Silencing on Bacterial Infection-induced ERK1/2 Signaling in Bone-marrow Derived Macrophages.  Bio-protocol, 8 (24).   ISSN 2331-8325