title: Macromolecular crowding-induced molten globule states of the alkali pH-denatured proteins.
creator: Kumar, Rajesh
creator: Kumar, Rajesh
creator: Sharma, Deepak
creator: Garg, Mansi
creator: Kumar, Vinay
creator: Agarwal, Mukesh Chand
subject: QR Microbiology
description: Structural and molecular properties extracted from circular dichroism (CD), tryptophan fluorescence and 1-anilino-8-napthalene sulfonate (ANS) binding experiments suggest that the high concentration of synthetic crowding agents (dextran 40, dextran 70 and ficoll 70) stabilizes and refolds the base-denatured ferricytochrome c (Ferricyt c) and lysozyme (Lyz) at pH 12.9 (±0.1) to molten globule (MG) states (C-states). These results further revealed that the C-states resemble the generic properties of MG-states. Thermodynamic analysis of thermal denaturation curves of base-denatured Ferricyt c and Lyz at pH 12.9 (±0.1) under variable concentrations of crowding agents (dextran 40, dextran 70 and ficoll 70) revealed that the crowder presence increases the thermal stability of base-denatured proteins and also prevents the cold denaturation of Ferricyt c. The results further showed that the nature, size and shape of crowder influence the crowding-mediated increase in secondary structure stabilization and thermal stability of base-denatured Ferricyt c and Lyz. Analysis of kinetic and thermodynamic parameters measured for CO association reaction of alkaline ferrocytochrome c (Ferrocyt c) at pH 12.9 (±0.1) under variable concentrations of crowding agents (dextran 40, dextran 70 and ficoll 70) revealed that the crowder presence reduces the level of structural fluctuation of M80-containing Ω-loop that control CO association to alkaline Ferrocyt c.
publisher: Elsevier Science
date: 2018-11-11
type: Article
type: PeerReviewed
relation: https://www.sciencedirect.com/science/article/pii/S157096391830147X?via%3Dihub
identifier:   Kumar, Rajesh and Kumar, Rajesh and Sharma, Deepak and Garg, Mansi and Kumar, Vinay and Agarwal, Mukesh Chand  (2018) Macromolecular crowding-induced molten globule states of the alkali pH-denatured proteins.  Biochimica et biophysica acta. Proteins and proteomics, 1866 (11).  pp. 1102-1114.  ISSN 1878-1454     
relation: http://crdd.osdd.net/open/2144/