<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "An F-type lectin domain directs the activity of Streptosporangium roseum alpha-l-fucosidase."^^ . "F-type lectins are phylogenetically widespread but selectively distributed fucose-binding lectins with L-fucose- and calcium-binding sequence motifs and an F-type lectin fold. Bacterial F-type lectin domains frequently occur in tandem with various protein domains in diverse architectures, indicating a possible role in directing enzyme activities or other biological functions to distinct fucosylated niches. Here, we report the biochemical characterization of a Streptosporangium roseum protein containing an F-type lectin domain in tandem with an NPCBM-associated domain and a family GH 29A alpha-l-fucosidase domain. We show that the F-type lectin domain of this protein recognizes fucosylated glycans in both α and β linkages but has high affinity for a Fuc-α-1,2-Gal motif and that the alpha-l-fucosidase domain displays hydrolytic activity on glycan substrates with α1-2 and α1-4 linked fucose. We also show that the F-type lectin domain does not have any effect on the activity of the cis-positioned alpha-l-fucosidase domain with the synthetic substrate, 4-Methylumbelliferyl-alpha-l-fucopyranoside or on inhibition of this activity by l-fucose or deoxyfuconojirimycin hydrochloride. However, the F-type lectin domain together with the NPCBM-associated domain enhances the activity of the cis-positioned alpha-l-fucosidase domain for soluble fucosylated oligosaccharide substrates. While there are many reports of glycoside hydrolase activity towards insoluble and soluble polysaccharides being enhanced by cis-positioned carbohydrate binding modules on the polypeptide, this is the first report, to our knowledge, of enhancement of activity towards aqueous, freely diffusible, small oligosaccharides. We propose a model involving structural stabilization and a bind-and-jump action mediated by the F-type lectin domain to rationalize our findings."^^ . "2018-11-01" . "28" . "11" . . "Oxford University Press"^^ . . . "Glycobiology"^^ . . . "14602423" . . . . . . . . . . . . . "T N C"^^ . "Ramya"^^ . "T N C Ramya"^^ . . "Sonal"^^ . "Mahajan"^^ . "Sonal Mahajan"^^ . . "Ritika"^^ . "Bishnoi"^^ . "Ritika Bishnoi"^^ . . . . . "HTML Summary of #2150 \n\nAn F-type lectin domain directs the activity of Streptosporangium roseum alpha-l-fucosidase.\n\n" . "text/html" . . . "QR Microbiology"@en . .