%0 Journal Article
%@ 1940-6029
%A Nagar, Rupa
%A Rao, Alka
%D 2019
%F open:2166
%I Springer
%J Methods in molecular biology (Clifton, N.J.)
%K Chymotrypsin digestion; Exoglycosidase treatment/Deglycosylation assay; Glycodiversification; High performance liquid chromatography (HPLC); In vitro glycosyltransferase assay; Mass spectrometry
%P 279-296
%T In Vitro Synthesis of Bioactive Glycovariants of Enterocin 96, an Antimicrobial Peptide from Enterococcus faecalis.
%U http://crdd.osdd.net/open/2166/
%V 1954
%X Antimicrobial peptides (AMPs) are novel agents for therapeutic application for their inherent broad spectrum of activities against bacteria, fungi, and viruses, as well as anti-inflammatory, anticancerous, and immunomodulatory activities. This chapter presents an enzymatic method to generate glycovariants of one such antimicrobial peptide, namely enterocin 96, using a bacterial protein O- and S-glycosyltransferase, in vitro.
%Z Copyright of this article belongs to Springer.