TY  - JOUR
N1  - Copyright of this article belongs to Springer.
ID  - open2166
UR  - https://link.springer.com/protocol/10.1007%2F978-1-4939-9154-9_22
A1  - Nagar, Rupa
A1  - Rao, Alka
Y1  - 2019/03/13/
N2  - Antimicrobial peptides (AMPs) are novel agents for therapeutic application for their inherent broad spectrum of activities against bacteria, fungi, and viruses, as well as anti-inflammatory, anticancerous, and immunomodulatory activities. This chapter presents an enzymatic method to generate glycovariants of one such antimicrobial peptide, namely enterocin 96, using a bacterial protein O- and S-glycosyltransferase, in vitro.
PB  - Springer
JF  - Methods in molecular biology (Clifton, N.J.)
VL  - 1954
KW  - Chymotrypsin digestion; Exoglycosidase treatment/Deglycosylation assay; Glycodiversification; High performance liquid chromatography (HPLC); In vitro glycosyltransferase assay; Mass spectrometry
SN  - 1940-6029
TI  - In Vitro Synthesis of Bioactive Glycovariants of Enterocin 96, an Antimicrobial Peptide from Enterococcus faecalis.
SP  - 279
EP  - 296
ER  -