%A Rupa Nagar
%A Alka Rao
%O Copyright of this article belongs to Springer.
%J Methods in molecular biology (Clifton, N.J.)
%T In Vitro Synthesis of Bioactive Glycovariants of Enterocin 96, an Antimicrobial Peptide from Enterococcus faecalis.
%X Antimicrobial peptides (AMPs) are novel agents for therapeutic application for their inherent broad spectrum of activities against bacteria, fungi, and viruses, as well as anti-inflammatory, anticancerous, and immunomodulatory activities. This chapter presents an enzymatic method to generate glycovariants of one such antimicrobial peptide, namely enterocin 96, using a bacterial protein O- and S-glycosyltransferase, in vitro.
%K Chymotrypsin digestion; Exoglycosidase treatment/Deglycosylation assay; Glycodiversification; High performance liquid chromatography (HPLC); In vitro glycosyltransferase assay; Mass spectrometry
%P 279-296
%V 1954
%D 2019
%I Springer
%L open2166