{
      "number": 2,
      "subjects": [
        "QR"
      ],
      "eprintid": 2182,
      "date": "2019-02-01",
      "userid": 2,
      "official_url": "https:\/\/academic.oup.com\/jb\/article\/165\/2\/125\/5146343",
      "issn": "1756-2651",
      "rev_number": 5,
      "creators": [
        {
          "name": {
            "lineage": null,
            "given": "Rishu",
            "honourific": null,
            "family": "Jain"
          }
        },
        {
          "name": {
            "lineage": null,
            "given": "Deepak",
            "honourific": null,
            "family": "Sharma"
          }
        },
        {
          "name": {
            "lineage": null,
            "given": "Rakesh",
            "honourific": null,
            "family": "Kumar"
          }
        },
        {
          "name": {
            "lineage": null,
            "given": "Rajesh",
            "honourific": null,
            "family": "Kumar"
          }
        }
      ],
      "dir": "disk0\/00\/00\/21\/82",
      "keywords": "alkali molten-globule, constrained dynamics, entropic stabilization, SDS, thermal stability",
      "lastmod": "2019-03-16 12:06:19",
      "ispublished": "pub",
      "pagerange": "125-137",
      "publisher": "Oxford University Press ",
      "metadata_visibility": "show",
      "date_type": "published",
      "eprint_status": "archive",
      "status_changed": "2019-03-16 12:06:19",
      "volume": 165,
      "datestamp": "2019-03-16 12:06:19",
      "uri": "http:\/\/crdd.osdd.net\/open\/id\/eprint\/2182",
      "note": "Copyright of this article belongs to OUP.",
      "refereed": "TRUE",
      "contact_email": "kps@imtech.res.in",
      "publication": "Journal of biochemistry",
      "abstract": "This study presents the structural, kinetic and thermodynamic characterizations of previously unknown submicellar concentrations of SDS-induced molten globule (MGSDS) state of a highly negatively charged base-denatured ferricytochrome c (UB-state) at pH ∼12.8 (±0.2). The far-UV CD, near-UV CD, ANS-fluorescence data of UB-state in the presence of different concentrations of SDS indicate that the submicellar concentrations of SDS (≤0.4 mM) transform the UB-state to MGSDS-state. The MGSDS-state has native-like α-helical secondary structure but lacks tertiary structure. The free energy change (ΔG°D) for UB→ MGSDS transition determined by far-UV CD (∼2.7 kcal mol-1) is slightly higher than those determined by fluorescence (∼2.0 kcal mol-1) at 25°C. At very low SDS and NaCl concentrations, the MGSDS-state undergoes cold denaturation. As SDS concentration is increased, the thermal denaturation temperature increases and the cold denaturation temperature decrease. Kinetic experiments involving the measurement of the CO-association rate to the base-denatured ferrocytochrome c at pH ≈12.8 (±0.2), 25°C indicate that the submicellar concentrations of SDS restrict the internal dynamics of base-denatured protein.",
      "type": "article",
      "title": "Structural, kinetic and thermodynamic characterizations of SDS-induced molten globule state of a highly negatively charged cytochrome c."
    }