TY  - JOUR
N1  - Copyright of this article belongs to OUP.
ID  - open2182
UR  - https://academic.oup.com/jb/article/165/2/125/5146343
IS  - 2
A1  - Jain, Rishu
A1  - Sharma, Deepak
A1  - Kumar, Rakesh
A1  - Kumar, Rajesh
Y1  - 2019/02/01/
N2  - This study presents the structural, kinetic and thermodynamic characterizations of previously unknown submicellar concentrations of SDS-induced molten globule (MGSDS) state of a highly negatively charged base-denatured ferricytochrome c (UB-state) at pH ?12.8 (±0.2). The far-UV CD, near-UV CD, ANS-fluorescence data of UB-state in the presence of different concentrations of SDS indicate that the submicellar concentrations of SDS (?0.4 mM) transform the UB-state to MGSDS-state. The MGSDS-state has native-like ?-helical secondary structure but lacks tertiary structure. The free energy change (?G°D) for UB? MGSDS transition determined by far-UV CD (?2.7 kcal mol-1) is slightly higher than those determined by fluorescence (?2.0 kcal mol-1) at 25°C. At very low SDS and NaCl concentrations, the MGSDS-state undergoes cold denaturation. As SDS concentration is increased, the thermal denaturation temperature increases and the cold denaturation temperature decrease. Kinetic experiments involving the measurement of the CO-association rate to the base-denatured ferrocytochrome c at pH ?12.8 (±0.2), 25°C indicate that the submicellar concentrations of SDS restrict the internal dynamics of base-denatured protein.
PB  - Oxford University Press 
JF  - Journal of biochemistry
VL  - 165
KW  - alkali molten-globule
KW  -  constrained dynamics
KW  -  entropic stabilization
KW  -  SDS
KW  -  thermal stability
SN  - 1756-2651
TI  - Structural, kinetic and thermodynamic characterizations of SDS-induced molten globule state of a highly negatively charged cytochrome c.
SP  - 125
EP  - 137
ER  -