TY  - JOUR
ID  - open234
UR  - http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2249.2003.02254.x/pdf
IS  - 1
A1  - Agrewala, J N
A1  - Suvas, S
A1  - Singh, V
A1  - Vohra, H
N2  - We have examined the possibility of evoking antigen-specific T cell immune response by using allogeneic cells as a source of adjuvant and also as a vehicle to deliver antigen. The mice were immunized with different preparations of antigen-pulsed allogeneic and syngeneic splenocytes. It was observed during the study that the animals immunized with antigen-pulsed mitomycin C treated allogeneic cells elicited antigen specific CD(4+) Th1 cell response. Predominant release of IL-2, interferon (IFN)-gamma and IgG2a-isotype also occurred. In contrast, mice immunized with antigen-pulsed syngeneic cells chiefly enhanced the production of interleukin (IL)-4 and IgG1-isotype. Further, allogeneic macrophages induced better T cell response than B cells or splenocytes and prominently induced the expression of B7-1 and B7-2. Immunization with antigen-pulsed macrophages provided better recall responses compared to B cells. This was manifested by the high LFA-1alpha and low CD45RB expression on T cells. Because it is already known that mitomycin C-treated cells undergo apoptosis and dendritic cells engulf apoptotic cells, we therefore propose that generation of T cell response using antigen-pulsed allogeneic cells may be due to the engulfment of these cells by dendritic cells, which may then process and present antigen entrapped in allogeneic cells to activate naive CD(4+) T cells and differentiate them to Th1 cells. This study therefore provides a rational basis for manipulating antigen-specific responses by immunizing with antigen-pulsed allogeneic cells.
VL  - 134
TI  - Delivery of antigen in allogeneic cells preferentially generates CD(4+) Th1 cells.
AV  - restricted
EP  - 22
N1  - Copyright of this article belongs to Wiley.
Y1  - 2003/10//
PB  - Wiley
JF  - Clinical and experimental immunology
KW  - allogeneic stimulation IFNg
IL-4 memory T cells Th1 cells Th2 cells
SN  - 0009-9104
SP  - 13
ER  -