creators_name: Agrewala, J N
creators_name: Suvas, S
creators_name: Singh, V
creators_name: Vohra, H
type: article
datestamp: 2012-01-06 14:57:19
lastmod: 2012-01-06 14:57:19
metadata_visibility: show
title: Delivery of antigen in allogeneic cells preferentially generates CD(4+) Th1 cells.
ispublished: pub
subjects: QR180
full_text_status: restricted
keywords: allogeneic stimulation IFNg
IL-4 memory T cells Th1 cells Th2 cells
note: Copyright of this article belongs to Wiley.
abstract: We have examined the possibility of evoking antigen-specific T cell immune response by using allogeneic cells as a source of adjuvant and also as a vehicle to deliver antigen. The mice were immunized with different preparations of antigen-pulsed allogeneic and syngeneic splenocytes. It was observed during the study that the animals immunized with antigen-pulsed mitomycin C treated allogeneic cells elicited antigen specific CD(4+) Th1 cell response. Predominant release of IL-2, interferon (IFN)-gamma and IgG2a-isotype also occurred. In contrast, mice immunized with antigen-pulsed syngeneic cells chiefly enhanced the production of interleukin (IL)-4 and IgG1-isotype. Further, allogeneic macrophages induced better T cell response than B cells or splenocytes and prominently induced the expression of B7-1 and B7-2. Immunization with antigen-pulsed macrophages provided better recall responses compared to B cells. This was manifested by the high LFA-1alpha and low CD45RB expression on T cells. Because it is already known that mitomycin C-treated cells undergo apoptosis and dendritic cells engulf apoptotic cells, we therefore propose that generation of T cell response using antigen-pulsed allogeneic cells may be due to the engulfment of these cells by dendritic cells, which may then process and present antigen entrapped in allogeneic cells to activate naive CD(4+) T cells and differentiate them to Th1 cells. This study therefore provides a rational basis for manipulating antigen-specific responses by immunizing with antigen-pulsed allogeneic cells.
date: 2003-10
date_type: published
publication: Clinical and experimental immunology
volume: 134
number: 1
publisher: Wiley
pagerange: 13-22
refereed: TRUE
issn: 0009-9104
official_url: http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2249.2003.02254.x/pdf
related_url_url: http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2249.2003.02254.x/pdf
related_url_type: pub
citation:   Agrewala, J N and Suvas, S and Singh, V and Vohra, H  (2003) Delivery of antigen in allogeneic cells preferentially generates CD(4+) Th1 cells.  Clinical and experimental immunology, 134 (1).  pp. 13-22.  ISSN 0009-9104     
document_url: http://crdd.osdd.net/open/234/1/vohra2003.pdf