%A Yash Pal
%A Shanmugam Mayilraj
%A Mohit Paul
%A Peter Schumann
%A Srinivasan Krishnamurthi
%O Copyright of this article belongs to Microbiology Society.
%J International journal of systematic and evolutionary microbiology
%T Indiicoccus explosivorum gen. nov., sp. nov., isolated from an explosives waste contaminated site.
%X A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19, was isolated from an explosives contaminated site in Panchkula, Haryana, India. The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13 (96.1?%), followed by Planococcus maitriensis S1 (95.6?%), Planococcus plakortidis DSM 23997 (95.6?%), Planomicrobium flavidum ISL-41 (95.6?%), Planococcus rifietoensis M8 (95.5?%), Planococcus salinus LCB217 (95.5?%) and Planococcus maritimus DSM 17275 (95.5?%). Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae. Strain S5-TSA-19 had a distinctive chemotaxonomic pattern comprising A4? type peptidoglycan based on l-Lys-d-Asp, iso-C15?:?0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny. Further, strain S5-TSA-19 was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4?days, whereas the closest reference strains did not possess this property. On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19 is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp. nov. is proposed (=JCM 31737=KCTC 33871=MTCC 12608).
%N 8
%K 16S rRNA gene; Indiicoccus; Indiicoccus explosivorum; S5-TSA-19T; biotransformation; explosives; phylogeny
%P 2555-2564
%V 69
%D 2019
%I Microbiology Society
%L open2340