creators_name: Pal, Yash
creators_name: Mayilraj, Shanmugam
creators_name: Paul, Mohit
creators_name: Schumann, Peter
creators_name: Krishnamurthi, Srinivasan
type: article
datestamp: 2019-08-13 14:57:46
lastmod: 2019-08-13 14:57:46
metadata_visibility: show
title: Indiicoccus explosivorum gen. nov., sp. nov., isolated from an explosives waste contaminated site.
ispublished: pub
subjects: QR
keywords: 16S rRNA gene; Indiicoccus; Indiicoccus explosivorum; S5-TSA-19T; biotransformation; explosives; phylogeny
note: Copyright of this article belongs to Microbiology Society.
abstract: A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19, was isolated from an explosives contaminated site in Panchkula, Haryana, India. The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13 (96.1 %), followed by Planococcus maitriensis S1 (95.6 %), Planococcus plakortidis DSM 23997 (95.6 %), Planomicrobium flavidum ISL-41 (95.6 %), Planococcus rifietoensis M8 (95.5 %), Planococcus salinus LCB217 (95.5 %) and Planococcus maritimus DSM 17275 (95.5 %). Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae. Strain S5-TSA-19 had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny. Further, strain S5-TSA-19 was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4 days, whereas the closest reference strains did not possess this property. On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19 is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp. nov. is proposed (=JCM 31737=KCTC 33871=MTCC 12608).
date: 2019-08
date_type: published
publication: International journal of systematic and evolutionary microbiology
volume: 69
number: 8
publisher: Microbiology Society
pagerange: 2555-2564
refereed: TRUE
issn: 1466-5034
official_url: https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003541#tab2
citation:   Pal, Yash and Mayilraj, Shanmugam and Paul, Mohit and Schumann, Peter and Krishnamurthi, Srinivasan  (2019) Indiicoccus explosivorum gen. nov., sp. nov., isolated from an explosives waste contaminated site.  International journal of systematic and evolutionary microbiology, 69 (8).  pp. 2555-2564.  ISSN 1466-5034