creators_name: Negi, Shikha
creators_name: Pahari, Susanta
creators_name: Das, Deepjyoti Kumar
creators_name: Khan, Nargis
creators_name: Agrewala, J N
type: article
datestamp: 2019-08-13 14:35:28
lastmod: 2019-08-13 14:35:28
metadata_visibility: show
title: Curdlan Limits  Survival Through STAT-1 Regulated Nitric Oxide Production.
ispublished: pub
subjects: QR180
keywords: T cells; curdlan; host-directed therapy; iNOS; macrophages; tuberculosis
note: Copyright of this article belongs to Frontiers Media S.A. 
abstract: Host-directed therapies have emerged as an innovative and promising approach in tuberculosis (TB) treatment due to the observed limitations of current TB regimen such as lengthy duration and emergence of drug resistance. Thus, we explored the role of curdlan (beta glucan polysaccharide) as a novel strategy to activate macrophages against  (). The aim of the study was to investigate the role of curdlan in restricting the  growth both  and . Further, the immunomodulatory potential of curdlan against  and the underlying mechanism is largely unknown. We found that curdlan treatment enhanced the antigen presentation, pro-inflammatory cytokines,  uptake and killing activity of macrophages.  studies showed that curdlan therapy significantly reduced the  burden in lung and spleen of mice. Administration of curdlan triggered the protective Th1 and Th17 immunity while boosting the central and effector memory response in  infected mice. Curdlan mediated anti- activity is through signal transducer and activator of transcription-1 (STAT-1), which regulates nitric oxide (NO) production through inducible NO synthase (iNOS) induction; along with this activation of nuclear factor kappa B (NF-κB) was also evident in  infected macrophages. Thus, we demonstrate that curdlan exerts effective anti-tuberculous activity anti-tuberculous activity. It can be used as a potential host-directed therapy against .
date: 2019
date_type: published
publication: Frontiers in microbiology
volume: 10
publisher: Frontiers Media S.A. 
pagerange: 1173
refereed: TRUE
issn: 1664-302X
official_url: https://www.frontiersin.org/articles/10.3389/fmicb.2019.01173/full
citation:   Negi, Shikha and Pahari, Susanta and Das, Deepjyoti Kumar and Khan, Nargis and Agrewala, J N  (2019) Curdlan Limits Survival Through STAT-1 Regulated Nitric Oxide Production.  Frontiers in microbiology, 10.  p. 1173.  ISSN 1664-302X