<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Carrier protein influences immunodominance of a known epitope: implication in peptide vaccine design."^^ . "We investigated how the processing of a given antigen by antigen presenting cells (APC) is dictated by the conformation of the antigen and how this governs the immunodominance hierarchy. To address the question, a known immunodominant sequence of bacteriophage lambda repressor N-terminal sequence 12-26 [λR(12-26)] was engineered at the N and C termini of a heterologous leishmanial protein, Kinetoplastid membrane protein-11 (KMP-11); the resulting proteins were defined as N-KMP-11 and C-KMP-11 respectively. The presence of λR(12-26) in N-KMP-11 and C-KMP-11 was established by western blot analysis with antibody to λR(12-26) peptide. N-KMP-11 but not C-KMP-11 could stimulate the anti λR(12-26) T-cell clonal population very efficiently in the presence of APCs. Priming of BALB/c mice with N-KMP-11 or C-KMP-11 generated similar levels of anti-KMP-11 IgG, but anti-λR(12-26) specific IgG was observed only upon priming with N-KMP-11. Interestingly, uptake of both N-KMP-11 and C-KMP-11 by APCs was similar but catabolism of N-KMP-11 but not C-KMP-11 was biphasic and fast at the initial time point. Kratky plots of small angle X-ray scattering showed that while N-KMP-11 adopts flexible Gaussian type of topology, C-KMP-11 prefers Globular nature. To show that KMP-11 is not unique as a carrier protein, an epitope (SPITBTNLBTMBK) of Plasmodium yoelii (PY) apical membrane protein 1[AMA-1 (136-148)], is placed at the C and N terminals of a dominant T-cell epitope of ovalbumin protein OVA(323-339) and the resulting peptides are defined as PY-OVA and OVA-PY respectively. Interestingly, only OVA-PY could stimulate anti-OVA T-cells and produce IgG response upon priming of BALB/c mice with it. Thus for rational design of peptide vaccine it is important to place the dominant epitope appropriately in the context of the carrier protein."^^ . "2013-09-23" . "31" . "41" . . "Elsevier Science"^^ . . . "Vaccine"^^ . . . "18732518" . . . . . . . . . . . . . . . . . . . . . . "Reema R"^^ . "Dhoke"^^ . "Reema R Dhoke"^^ . . "Syamal"^^ . "Roy"^^ . "Syamal Roy"^^ . . "Ashish K"^^ . "Solanki"^^ . "Ashish K Solanki"^^ . . "."^^ . "Ashish"^^ . ". Ashish"^^ . . "Koushik"^^ . "Roy"^^ . "Koushik Roy"^^ . . "Moumita"^^ . "Ghosh"^^ . "Moumita Ghosh"^^ . . . . . "HTML Summary of #2438 \n\nCarrier protein influences immunodominance of a known epitope: implication in peptide vaccine design.\n\n" . "text/html" . . . "QR Microbiology"@en . .