title: UV-FIA: UV-induced fluoro-immunochemical assay for ultra-trace detection of PETN, RDX, and TNT.
creator: Chaudhary, Shilpa
creator: Sonkusre, Praveen
creator: Chopra, Adity
creator: Bhasin, K K
creator: Suri, C Raman
subject: QR Microbiology
description: Fluorescence quenching based immunoassay format for the detection of a trace amount of some nitro-explosives with a high degree of selectivity is reported in this study. The immunoassay comprises anti-explosive antibodies functionalized microtitre strips specific to the targeted explosives, pentaerythritol tetranitrate (PETN), 1,3,5-trinitroperhydro-1,3,5-triazine (RDX), and 2,4,6-trinitrotoluene (TNT). UV induced photolysis of nitro-explosive bound to targeted antibodies generates primarily nitrite ions which after the quick reaction with the detector molecule, 2,3-diaminonaphthalene (DAN), a fluorophore, quenches its fluorescence intensity, however, proportionately undergo cyclization to produce a highly fluorescent product, 2,3-naphthotriazole (NAT). The synthesized product, NAT, was verified using various chromatographic and spectrophotometric techniques. This newly developed antibody-based detection method, utilizing DAN dye, demonstrated a high selectivity towards PETN, RDX, and TNT. This method can be used as an economical testing kit for direct quantification of explosives, implying the great potential for quick, low-cost trace detection of explosives.
publisher: Elsevier Science
date: 2019-10-24
type: Article
type: PeerReviewed
relation: https://www.sciencedirect.com/science/article/pii/S0003267019306452?via%3Dihub
identifier:   Chaudhary, Shilpa and Sonkusre, Praveen and Chopra, Adity and Bhasin, K K and Suri, C Raman  (2019) UV-FIA: UV-induced fluoro-immunochemical assay for ultra-trace detection of PETN, RDX, and TNT.  Analytica chimica acta, 1077.  pp. 266-272.  ISSN 1873-4324     
relation: http://crdd.osdd.net/open/2497/