<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31."^^ . "DivIVA or Wag31, which is an essential pole organizing protein in mycobacteria, can self-assemble at the negatively curved side of the membrane at the growing pole to form a higher order structural scaffold for maintaining cellular morphology and localizing various target proteins for cell-wall biogenesis. The structural organization of polar scaffold formed by polymerization of coiled-coil rich Wag31, which is implicated in the anti-tubercular activities of amino-pyrimidine sulfonamides, remains to be determined. A single-site phosphorylation in Wag31 regulates peptidoglycan biosynthesis in mycobacteria. We report biophysical characterizations of filaments formed by mycobacterial Wag31 using circular dichroism, atomic force microscopy and small angle solution X-ray scattering. Atomic force microscopic images of the wild-type, a phospho-mimetic (T73E) and a phospho-ablative (T73A) form of Wag31 show mostly linear filament formation with occasional curving, kinking and apparent branching. Solution X-ray scattering data indicates that the phospho-mimetic forms of the Wag31 polymers are on average more compact than their phospho-ablative counterparts, which is likely due to the extent of bending/branching. Observed structural features in this first view of Wag31 filaments suggest a basis for higher order Wag31 scaffold formation at the pole."^^ . "2019-11-25" . . "Elsevier Science "^^ . . . "Journal of structural biology"^^ . . . "10958657" . . . . . . . . . . . . . . . . . . . . . . "Barnali"^^ . "Chaudhuri"^^ . "Barnali Chaudhuri"^^ . . "Munia"^^ . "Ganguli"^^ . "Munia Ganguli"^^ . . "Karman"^^ . "Virk"^^ . "Karman Virk"^^ . . "Aanchal"^^ . "Gupta"^^ . "Aanchal Gupta"^^ . . "Brohmomoy"^^ . "Basu"^^ . "Brohmomoy Basu"^^ . . "Komal"^^ . "Choukate"^^ . "Komal Choukate"^^ . . . . . "HTML Summary of #2503 \n\nHigher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.\n\n" . "text/html" . . . "QR Microbiology"@en . .