creators_name: Choukate, Komal
creators_name: Gupta, Aanchal
creators_name: Basu, Brohmomoy
creators_name: Virk, Karman
creators_name: Ganguli, Munia
creators_name: Chaudhuri, Barnali
type: article
datestamp: 2019-12-06 15:41:35
lastmod: 2019-12-06 15:41:35
metadata_visibility: show
title: Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.
ispublished: pub
subjects: QR
keywords: AFM; DivIVA; Mycobacterial growth; SAXS; Wag31 filament; bacterial polar growth


note: Copyright of this article belongs to Elsevier Science 
abstract: DivIVA or Wag31, which is an essential pole organizing protein in mycobacteria, can self-assemble at the negatively curved side of the membrane at the growing pole to form a higher order structural scaffold for maintaining cellular morphology and localizing various target proteins for cell-wall biogenesis. The structural organization of polar scaffold formed by polymerization of coiled-coil rich Wag31, which is implicated in the anti-tubercular activities of amino-pyrimidine sulfonamides, remains to be determined. A single-site phosphorylation in Wag31 regulates peptidoglycan biosynthesis in mycobacteria. We report biophysical characterizations of filaments formed by mycobacterial Wag31 using circular dichroism, atomic force microscopy and small angle solution X-ray scattering. Atomic force microscopic images of the wild-type, a phospho-mimetic (T73E) and a phospho-ablative (T73A) form of Wag31 show mostly linear filament formation with occasional curving, kinking and apparent branching. Solution X-ray scattering data indicates that the phospho-mimetic forms of the Wag31 polymers are on average more compact than their phospho-ablative counterparts, which is likely due to the extent of bending/branching. Observed structural features in this first view of Wag31 filaments suggest a basis for higher order Wag31 scaffold formation at the pole.
date: 2019-11-25
date_type: published
publication: Journal of structural biology
publisher: Elsevier Science 
pagerange: 107429
refereed: TRUE
issn: 1095-8657
official_url: https://www.sciencedirect.com/science/article/pii/S1047847719302552?via%3Dihub
citation:   Choukate, Komal and Gupta, Aanchal and Basu, Brohmomoy and Virk, Karman and Ganguli, Munia and Chaudhuri, Barnali  (2019) Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.  Journal of structural biology.  p. 107429.  ISSN 1095-8657