TY  - JOUR
N1  - Copyright of article belongs to International Union of Microbiological Societies.
ID  - open2575
UR  - https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004222#tab2
A1  - Verma, Ashish
A1  - Pal, Yash
A1  - Kumar, Pravin
A1  - Krishnamurthi, Srinivasan
Y1  - 2020/05/18/
N2  - A novel archaeal strain designated as SPP-AMP-1T was isolated from saltpan soil, using the serial dilution method on a halophilic archaeal medium supplemented with ampicillin. Cells were both rod-shaped and pleomorphic in nature, non-motile, unable to produce acid from a variety of sugars or grow anaerobically with different substrates (l-arginine) and electron acceptors (DMSO, nitrate). Optimal growth was observed at 42 °C, 3.4-4.2 M NaCl and pH 7.2. Cells did not lyse in distilled water and grew in the absence of Mg2+ ions. Phylogenetic analysis based on the sequences of 16S rRNA gene, amino acid sequence of ?'-subunit of RNA polymerase and 400 conserved proteins retrieved from the whole genome assemblies showed that strain SPP-AMP-1T was distantly related to any existing genera within the family Halobacteriaceae. MK-8 was the only quinone detected. Polar lipid analysis showed a unique combination of diethyl derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, glycosyl-mannosyl-glucosyl diether and sulphated glycosyl-mannosyl-glucosyl diether as the major lipids. The G+C content of genomic DNA is 57.7 mol%. The phenotypic, phylogenetic and genomic data supported the concept of the novel genus status of strain SPP-AMP-1T in the family Halobacteriaceae for which the name Halocatena pleomorpha gen. nov., sp. nov., is proposed; the type strain is SPP-AMP-1T (=JCM 31368T=KCTC 4276T=MTCC 12579T).
PB  - International Union of Microbiological Societies 
JF  - International journal of systematic and evolutionary microbiology
KW  - 16S rRNA; Halobacteriaceae; SPP-AMP-1T
SN  - 1466-5034
TI  - Halocatena pleomorpha Gen. Nov. Sp. Nov., an Extremely Halophilic Archaeon of Family Halobacteriaceae Isolated From Saltpan Soil.
ER  -