@article{open2584,
           month = {July},
          author = {Pallavi Ahirrao and Rushikesh Tambat and Nishtha Chandal and Nisha Mahey and Anjoo Kamboj and Upendra K. Jain and Inder Pal Singh and Sanjay M. Jachak and Hemraj Nandanwar},
            note = {Copyright of this article belongs to WILEY-V C H VERLAG.},
         address = {POSTFACH 101161, 69451 WEINHEIM, GERMANY},
           title = {MsrA Efflux Pump Inhibitory Activity ofPiper cubebaL.f. and its Phytoconstituents againstStaphylococcus aureusRN4220},
       publisher = {WILEY-V C H VERLAG GMBH},
         journal = {Chemistry \& Biodiversity},
            year = {2020},
        keywords = {Piper cubeba; methicillin-resistantStaphylococcus aureus(MRSA); MsrA efflux pump; pellitorine; sesamin; phytoconstituents},
             url = {http://crdd.osdd.net/open/2584/},
        abstract = {MsrA, an efflux pump belonging to ATP-binding cassette (ABC) transporter family that conferred resistance to macrolides, was detected inStaphylococcus aureusstrains. Herein, we report the isolation of phytoconstituents fromPiper cubebafruit methanol extract and investigated their efflux pump inhibitory potential againstS. aureusMsrA pump. Four isolated compounds, viz. pellitorine, sesamin, piperic acid and tetrahydropiperine studied in combination with erythromycin inS. aureusRN4220, exhibited 2-8-fold reduction in minimum inhibitory concentration (MIC) of erythromycin. Pellitorine and sesamin decreased MIC of erythromycin by 8-fold. The real-time fluorometry-based efflux and accumulation studies of ethidium bromide (EtBr) onS. aureusRN4220 in the presence of these compounds showed reduced efflux and enhanced uptake, thus indicating inhibition of the efflux pump. Pellitorine showed significant post-antibiotic effect of erythromycin. The results revealed that the primary mechanism of action of these compounds involves steady ATP production impairment.}
}