title: MsrA Efflux Pump Inhibitory Activity ofPiper cubebaL.f. and its Phytoconstituents againstStaphylococcus aureusRN4220
creator: Ahirrao, Pallavi
creator: Tambat, Rushikesh
creator: Chandal, Nishtha
creator: Mahey, Nisha
creator: Kamboj, Anjoo
creator: Jain, Upendra K.
creator: Singh, Inder Pal
creator: Jachak, Sanjay M.
creator: Nandanwar, Hemraj
subject: QR Microbiology
description: MsrA, an efflux pump belonging to ATP-binding cassette (ABC) transporter family that conferred resistance to macrolides, was detected inStaphylococcus aureusstrains. Herein, we report the isolation of phytoconstituents fromPiper cubebafruit methanol extract and investigated their efflux pump inhibitory potential againstS. aureusMsrA pump. Four isolated compounds, viz. pellitorine, sesamin, piperic acid and tetrahydropiperine studied in combination with erythromycin inS. aureusRN4220, exhibited 2-8-fold reduction in minimum inhibitory concentration (MIC) of erythromycin. Pellitorine and sesamin decreased MIC of erythromycin by 8-fold. The real-time fluorometry-based efflux and accumulation studies of ethidium bromide (EtBr) onS. aureusRN4220 in the presence of these compounds showed reduced efflux and enhanced uptake, thus indicating inhibition of the efflux pump. Pellitorine showed significant post-antibiotic effect of erythromycin. The results revealed that the primary mechanism of action of these compounds involves steady ATP production impairment.
publisher: WILEY-V C H VERLAG GMBH
date: 2020-07-05
type: Article
type: PeerReviewed
relation: https://onlinelibrary.wiley.com/doi/full/10.1002/cbdv.202000144
identifier:   Ahirrao, Pallavi and Tambat, Rushikesh and Chandal, Nishtha and Mahey, Nisha and Kamboj, Anjoo and Jain, Upendra K. and Singh, Inder Pal and Jachak, Sanjay M. and Nandanwar, Hemraj  (2020) MsrA Efflux Pump Inhibitory Activity ofPiper cubebaL.f. and its Phytoconstituents againstStaphylococcus aureusRN4220.  Chemistry & Biodiversity.      (In Press)  
relation: http://crdd.osdd.net/open/2584/