@article{open2640, volume = {78}, month = {May}, author = {Narender Kumar and Sarabjeet Kour Sudan and Roma Garg and Girish Sahni}, note = {The copyright of this article belongs to Elsevier Science.}, title = {Enhanced production of novel halostable recombinant endoglucanase derived from the metagenomic library using fed-batch fermentation.}, publisher = {Elsevier Science}, journal = {Process Biochemistry}, pages = {1--7}, year = {2019}, keywords = {Endoglucanase;Halostable;Metagenomic;Fed-batch fermentation}, url = {http://crdd.osdd.net/open/2640/}, abstract = {Novel endoglucanase was isolated from soil metagenomic library. 40 fold increase in yield was obtained using fed- batch strategies as compared to shake flask. Purified endogluconase was halostable and halotolerant as it retained 100\% activity after 30 days incubation in presence of salts. The purified enzyme was also found to be stable in presence of ions, organic solvents, surfactant and detergents.} }