creators_name: Chaudhari, Ramesh
creators_name: Tandel, Nikunj
creators_name: Sahu, Kiran
creators_name: Negi, Sushmita
creators_name: Bashir, Hilal
creators_name: Rupareliya, Arzu
creators_name: Mishra, Ravi Pn
creators_name: Dalai, Sarat K.
creators_name: Tyagi, Rajeev K
type: article
datestamp: 2021-03-26 04:11:21
lastmod: 2021-03-26 04:11:21
metadata_visibility: show
title: Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of P. falciparum (PfMSP-Fu(24)) Mounts Protective Immune Response
ispublished: pub
subjects: QR
keywords: PfMSP-Fu24; elastic liposomes; humoral and cellular immunity; malaria; vaccine.
note: Open Access
abstract: Transdermal immunization exhibits poor immunogenic responses due to poor permeability of antigens through the skin. Elastic liposomes, the ultradeformable nanoscale lipid vesicles, overcome the permeability issues and prove a versatile nanocarrier for transcutaneous delivery of protein, peptide, and nucleic acid antigens. Elastic liposome-mediated subcutaneous delivery of chimeric fusion protein (PfMSP-Fu(24)) of Plasmodium falciparum exhibited improved immunogenic responses. Elastic liposomes-mediated immunization of PfMSP-Fu(24) conferred immunity to the asexual blood-stage infection. Present study is an attempt to compare the protective immune response mounted by the PfMSP-Fu(24) upon administered through transdermal and intramuscular routes. Humoral and cell-mediated immune (CMI) response elicited by topical and intramuscularly administered PfMSP-Fu(24)-laden elastic liposomes (EL-PfMSP-Fu(24)) were compared and normalized with the vehicle control. Sizeable immune responses were seen with the transcutaneously immunized EL-PfMSP-Fu(24) and compared with those elicited with intramuscularly administered antigen. Our results show significant IgG isotype subclass (IgG1and IgG3) response of specific antibody levels as well as cell-mediated immunity (CMI) activating factor (IFN-gamma), a crucial player in conferring resistance to blood-stage malaria in mice receiving EL-PfMSP-Fu(24) through transdermal route as compared to the intramuscularly administered formulation. Heightened immune response obtained by the vaccination of EL-PfMSP-Fu(24) was complemented by the quantification of the transcript (mRNA) levels cell-mediated (IFN-gamma, IL-4), and regulatory immune response (IL-10) in the lymph nodes and spleen. Collectively, elastic liposomes prove their immune-adjuvant property as they evoke sizeable and perdurable immune response against PfMSP-Fu(24) and justify its potential for the improved vaccine delivery to inducing both humoral and CM immune response.
date: 2021-02-05
date_type: published
publication: Nanomaterials
volume: 11
number: 2
publisher: MDPI
pagerange: 1-23
refereed: TRUE
official_url: https://www.mdpi.com/2079-4991/11/2/406
citation:   Chaudhari, Ramesh and Tandel, Nikunj and Sahu, Kiran and Negi, Sushmita and Bashir, Hilal and Rupareliya, Arzu and Mishra, Ravi Pn and Dalai, Sarat K. and Tyagi, Rajeev K  (2021) Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of P. falciparum (PfMSP-Fu(24)) Mounts Protective Immune Response.  Nanomaterials, 11 (2).  pp. 1-23.