TY  - JOUR
N1  - Copyright of this article belongs to Elsevier Science/Academic Press
ID  - open2697
UR  - https://www.sciencedirect.com/science/article/abs/pii/S0147619X21000044?via%3Dihub
A1  - Srivastava, Suchita
A1  - Kaur, Satinderdeep
A1  - Verma, Hemant Kumar
A1  - Rani, Suman
A1  - Thakur, Manisha
A1  - Haldar, Swati
A1  - Singh, Jagmohan
Y1  - 2021/05//
N2  - The fission yeast, Schizosaccharomyces pombe, is an excellent model for basic research but is not useful for commercial scale protein expression due to lack of strong expression vectors. Earlier, we showed that the lsd90 promoter elicited significantly greater GFP expression level than the adh1 and nmt1 promoters, albeit in different vector backbones. Here, we have systematically investigated the contribution of selectable markers, LEU2 and URA3m to GFP expression: while LEU2 elicited very low expression, the URA3m gene, with truncated promoter, elicited much greater GFP expression level with all promoters. Paradoxically, an inverse correlation was observed between the GFP transcription and translation efficiency. This system can be useful for understanding the factors governing recombinant gene expression and optimization of protein production.
PB  - Elsevier Science/Academic Press
JF  - Plasmid
VL  - 115
KW  - Reporter gene; Promoter; Selectable marker; Transcription; Translation; GFP
SN  - 1095-9890
TI  - Reciprocal relation between reporter gene transcription and translation efficiency in fission yeast
ER  -