creators_name: Jadon, Rajesh Singh
creators_name: Sharma, Gajanand
creators_name: Garg, Neeraj
creators_name: Tandel, Nikunj
creators_name: Gajbhiye, Kavita 
creators_name: Salve, Rajesh
creators_name: Gajbhiye, Virendra
creators_name: Sharma, Ujjawal
creators_name: Katare, O P
creators_name: Sharma, Manoj
creators_name: Tyagi, Rajeev K
type: article
datestamp: 2021-06-29 08:39:33
lastmod: 2021-06-29 08:39:33
metadata_visibility: show
title: Efficient in vitro and in vivo docetaxel delivery mediated by pH-sensitive LPHNPs for effective breast cancer therapy
ispublished: pub
subjects: QR
keywords: Breast cancer; Docetaxel; Cytosolic delivery; Apoptosis; pH sensitive; Lipid polymer hybrid nanoparticles
note: Copyright of this article belongs to Elsevier Science/Academic Press
abstract: The present study was designed to develop pH-sensitive lipid polymer hybrid nanoparticles (pHS-LPHNPs) for specific cytosolic-delivery of docetaxel (DTX). The pHS-LPHNPs-DTX formulation was prepared by self assembled nano-precipitation technique and characterized for zeta potential, particle size, entrapment efficiency, polydispersity index (PDI), and in vitro drug release. In vitro cytotoxicity of pHS-LPHNPs-DTX was assessed on breast cancer cells (MDA-MB-231 and MCF-7) and compared with DTX-loaded conventional LPHNPs and bare DTX. In vitro cellular uptake in MDA-MB-231 cell lines showed better uptake of pHS-LPHNPs. Further, a significant reduction in the IC50 of pHS-LPHNPs-DTX against both breast cancer cells was observed. Flow cytometry results showed greater apoptosis in case of pHS-LPHNPs-DTX treated MDA-MB-231 cells. Breast cancer was experimentally induced in BALB/c female mice, and the in vivo efficacy of the developed pHS-LPHNPs formulation was assessed with respect to the pharmacokinetics, biodistribution in the vital organs (liver, kidney, heart, lungs, and spleen), percentage tumor burden, and survival of breast cancer-bearing animals. In vivo studies showed improved pharmacokinetic and target-specificity with minimum DTX circulation in the deep-seated organs in the case of pHS-LPHNPs-DTX compared to the LPHNPs-DTX and free DTX. Mice treated with pHSLPHNPs-DTX exhibited a significantly lesser tumor burden than other treatment groups. Also, reduced distribution of DTX in the serum was evident for pHS-LPHNPs-DTX treated mice compared to the LPHNPs-DTX and free DTX. In essence, pHS-LPHNPs mediated delivery of DTX presents a viable platform for developing therapeutic-interventions against breast-cancer.
date: 2021-07
date_type: published
publication: COLLOIDS AND SURFACES B-BIOINTERFACES
volume: 203
publisher: Elsevier Science/Academic Press
refereed: TRUE
issn: 0927-7765
official_url: https://www.sciencedirect.com/science/article/abs/pii/S0927776521002046
citation:   Jadon, Rajesh Singh and Sharma, Gajanand and Garg, Neeraj and Tandel, Nikunj and Gajbhiye, Kavita  and Salve, Rajesh and Gajbhiye, Virendra and Sharma, Ujjawal and Katare, O P and Sharma, Manoj and Tyagi, Rajeev K  (2021) Efficient in vitro and in vivo docetaxel delivery mediated by pH-sensitive LPHNPs for effective breast cancer therapy.  COLLOIDS AND SURFACES B-BIOINTERFACES, 203.   ISSN 0927-7765