<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Chaperone-assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM 10."^^ . "The N-carbamoyl-D-amino acid amidohydrolase (D-carbamoylase) gene (dcb) from Agrobacterium tumefaciens AM 10 was cloned by polymerase chain reaction in plasmid pET28a and was overexpressed in Escherichia coli JM109 (DE3). However, almost 80% of the enzyme remained trapped in inclusion bodies. To facilitate the expression of the properly folded active enzyme, the chaperones GroEL/ES were coexpressed in plasmid pKY206. This resulted in a 43-fold increase in active enzyme production compared to the wild-type strain. The histidyl-tagged D-carbamoylase was purified by a single step nickel-affinity chromatography to a specific activity of 9.5 U/mg protein."^^ . "2001-12" . . "23" . "3" . . "Elsevier Science"^^ . . . "Protein expression and purification"^^ . . . "10465928" . . . . . . . . . . . . . "R M"^^ . "Vohra"^^ . "R M Vohra"^^ . . "R"^^ . "Sharma"^^ . "R Sharma"^^ . . "D"^^ . "Sareen"^^ . "D Sareen"^^ . . . . . . "Chaperone-assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM 10. (PDF)"^^ . . . . . "Chaperone-assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM 10. (Image (PNG))"^^ . . . . . . "Chaperone-assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM 10. (Image (PNG))"^^ . . . . . . "Chaperone-assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM 10. (Image (PNG))"^^ . . . . . . "Chaperone-assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM 10. (Image (PNG))"^^ . . . . . . "Chaperone-assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM 10. (Indexer Terms)"^^ . . . . . "HTML Summary of #282 \n\nChaperone-assisted overexpression of an active D-carbamoylase from Agrobacterium tumefaciens AM 10.\n\n" . "text/html" . . . "QR Microbiology"@en . .