…
comment: The repository administrator has not yet configured an RDF license.
primary topic: http://crdd.osdd.net/open/id/eprint/297

http://crdd.osdd.net/open/id/eprint/297
type: AcademicArticle
type: Article
title: Interferon-gamma- and lipopolysaccharide-induced tumor necrosis factor-alpha is required for nitric oxide production: tumor necrosis factor-alpha and nitric oxide are independently involved in the killing of Mycobacterium microti in interferon-gamma- and lipopolysaccharide-treated J774A.1 cells.
abstract: A comparative study was done using J774A.1 and J774A.1-derived transfected cells (J774A.1 C.1) containing antisense tumor necrosis factor alpha (TNF-alpha) plasmid to determine the role of endogenous TNF-alpha on nitric oxide production as well as on the growth of Mycobacterium microti in interferon gamma (IFN-gamma)- and lipopolysaccharide (LPS)-treated cells. On stimulation with IFN-gamma and LPS a higher level of NO was observed in J774A.1 cells compared to J774A.1 C.1 which indicated that endogenous TNF-alpha is required for the production of NO. Comparing the effect of IFN-gamma and LPS on the intracellular growth of M. microti, the growth-reducing activity was higher in J774A.1 cells than in J774A.1 C.1 cells and was not completely abrogated in the presence of the nitric oxide inhibitor NG-methyl-L-arginine (L-NMA). J774A.1 C.1 cells infected with M. microti produced a significant amount of NO when exogenous TNF-alpha was added along with IFN-gamma and LPS and the concentration of intracellular bacteria decreased almost to that in IFN-gamma and LPS treated parental J774A.1 cells. Addition of exogenous TNF-alpha even in the presence of L-NMA in J774A.1 C.1 cells could also partially restore intracellular growth inhibition of M. microti caused by IFN-gamma and LPS. TNF-alpha is probably required for the production of NO in J774A.1 cells by IFN-gamma and LPS but TNF-alpha and NO are independently involved in the killing of intracellular M. microti with IFN-gamma and LPS.
date: 2000
volume: 45
issue: 5
is part of: Folia microbiologica
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: http://crdd.osdd.net/open/id/document/1553
has version: http://crdd.osdd.net/open/id/document/1553
has published: http://crdd.osdd.net/open/id/document/1553
has document: http://crdd.osdd.net/open/id/document/1554
has document: http://crdd.osdd.net/open/id/document/1555
has document: http://crdd.osdd.net/open/id/document/1556
has document: http://crdd.osdd.net/open/id/document/1557
has document: http://crdd.osdd.net/open/id/document/1558
subject: QR Microbiology

http://crdd.osdd.net/open/id/document/1553
type: Document
label: Interferon-gamma- and lipopolysaccharide-induced tumor necrosis factor-alpha is required for nitric oxide production: tumor necrosis factor-alpha and nitric oxide are independently involved in the killing of Mycobacterium microti in interferon-gamma- and lipopolysaccharide-treated J774A.1 cells. (PDF)

Springer Science
type: Organization

Folia microbiologica
type: Collection
issn: 00155632

S Majumdar
type: Person
given name: S
family name: Majumdar

R Gupta
type: Person
given name: R
family name: Gupta

N Dogra
type: Person
given name: N
family name: Dogra

http://crdd.osdd.net/open/id/document/1554
type: Document
label: Interferon-gamma- and lipopolysaccharide-induced tumor necrosis factor-alpha is required for nitric oxide production: tumor necrosis factor-alpha and nitric oxide are independently involved in the killing of Mycobacterium microti in interferon-gamma- and lipopolysaccharide-treated J774A.1 cells. (Image (PNG))
is version of: http://crdd.osdd.net/open/id/document/1553
is volatile version of: http://crdd.osdd.net/open/id/document/1553
islightbox thumbnail version of: http://crdd.osdd.net/open/id/document/1553

http://crdd.osdd.net/open/id/document/1555
type: Document
label: Interferon-gamma- and lipopolysaccharide-induced tumor necrosis factor-alpha is required for nitric oxide production: tumor necrosis factor-alpha and nitric oxide are independently involved in the killing of Mycobacterium microti in interferon-gamma- and lipopolysaccharide-treated J774A.1 cells. (Image (PNG))
is version of: http://crdd.osdd.net/open/id/document/1553
is volatile version of: http://crdd.osdd.net/open/id/document/1553
ispreview thumbnail version of: http://crdd.osdd.net/open/id/document/1553

http://crdd.osdd.net/open/id/document/1556
type: Document
label: Interferon-gamma- and lipopolysaccharide-induced tumor necrosis factor-alpha is required for nitric oxide production: tumor necrosis factor-alpha and nitric oxide are independently involved in the killing of Mycobacterium microti in interferon-gamma- and lipopolysaccharide-treated J774A.1 cells. (Image (PNG))
is version of: http://crdd.osdd.net/open/id/document/1553
is volatile version of: http://crdd.osdd.net/open/id/document/1553
ismedium thumbnail version of: http://crdd.osdd.net/open/id/document/1553

http://crdd.osdd.net/open/id/document/1557
type: Document
label: Interferon-gamma- and lipopolysaccharide-induced tumor necrosis factor-alpha is required for nitric oxide production: tumor necrosis factor-alpha and nitric oxide are independently involved in the killing of Mycobacterium microti in interferon-gamma- and lipopolysaccharide-treated J774A.1 cells. (Image (PNG))
is version of: http://crdd.osdd.net/open/id/document/1553
is volatile version of: http://crdd.osdd.net/open/id/document/1553
issmall thumbnail version of: http://crdd.osdd.net/open/id/document/1553

http://crdd.osdd.net/open/id/document/1558
type: Document
label: Interferon-gamma- and lipopolysaccharide-induced tumor necrosis factor-alpha is required for nitric oxide production: tumor necrosis factor-alpha and nitric oxide are independently involved in the killing of Mycobacterium microti in interferon-gamma- and lipopolysaccharide-treated J774A.1 cells. (Indexer Terms)
is version of: http://crdd.osdd.net/open/id/document/1553
is volatile version of: http://crdd.osdd.net/open/id/document/1553
is index codes version of: http://crdd.osdd.net/open/id/document/1553

http://crdd.osdd.net/open/297/
title: HTML Summary of #297 Interferon-gamma- and lipopolysaccharide-induced tumor necrosis factor-alpha is required for nitric oxide production: tumor necrosis factor-alpha and nitric oxide are independently involved in the killing of Mycobacterium microti in interferon-gamma- and lipopolysaccharide-treated J774A.1 cells.
format: text/html
primary topic: http://crdd.osdd.net/open/id/eprint/297

QR Microbiology
type: Concept